二甲双胍对甲状腺功能减退大鼠甲状腺激素的影响

发布时间：2018-02-03 00:04

本文关键词： 甲状腺二甲双胍甲状腺激素类甲状腺功能减退症钠碘同向转运体　出处：《第二军医大学学报》2017年05期 　论文类型：期刊论文

【摘要】：目的探讨二甲双胍对甲状腺功能减退大鼠甲状腺激素水平的影响及其机制。方法取3个月龄雄性SD大鼠36只,随机分为正常对照(NC)组、甲状腺功能减退(HT)组、二甲双胍(Met)组。HT组和Met组以含0.01%氨基三唑的饮用水喂养大鼠4周制备甲状腺功能减退大鼠模型,NC组大鼠喂养普通饮用水。造模成功后,Met组用200 mg/kg二甲双胍灌胃,HT组和NC组用等体积0.05%羟甲基纤维素钠(CMC-Na)灌胃。于灌胃第8周时对3组大鼠甲状腺组织行H-E染色,采用q PCR检测下丘脑促甲状腺激素释放激素前体(pro TRH)mRNA的表达;于灌胃第8、12周时采用电化学发光法检测3组大鼠血清T3、T4水平,采用q PCR和蛋白质印迹法检测甲状腺组织钠碘同向转运体(NIS)mRNA和蛋白表达。结果在灌胃8、12周时,HT组大鼠血清T3、T4水平均低于NC组,而Met组T3、T4水平均高于HT组(P0.05)。灌胃8周时,H-E染色结果显示NC组滤泡大小不等,内见大量胶质及吸收空泡;HT组大鼠甲状腺滤泡缩小,滤泡上皮成柱状,滤泡周围吸收空泡减少;Met组滤泡上皮增生较HT组稍改善。灌胃8周时,q PCR结果显示HT组大鼠下丘脑pro TRH和甲状腺组织NIS mRNA表达均高于NC组,而Met组均低于HT组(P0.05);蛋白质印迹法检测结果显示Met组NIS蛋白表达低于HT组(P0.05),而HT组和NC组NIS蛋白表达差异无统计学意义(P0.05)。灌胃12周时,HT组和Met组甲状腺组织NIS mRNA表达差异无统计学意义(P0.05),Met组NIS蛋白表达高于HT组(P0.05)。结论二甲双胍能升高甲状腺功能减退大鼠的甲状腺激素水平,但短期内并不是由甲状腺NIS表达升高所致。
[Abstract]:Objective to investigate the effect and mechanism of metformin on thyroid hormone level in hypothyroidism rats. Methods Thirty-six male SD rats aged 3 months were randomly divided into normal control group (n = 36). Hypothyroidism group, metformin metformin group, HT group and Met group were fed with drinking water containing 0.01% aminotriazole for 4 weeks to establish hypothyroidism rat model. Rats in NC group were fed with common drinking water. After the model was established, rats in Met group were fed with metformin for 200 mg/kg. HT and NC groups were fed with 0.05% hydroxymethyl cellulose sodium (CMC-Na) in the same volume. H-E staining was performed on the thyroid tissues of the three groups at the 8th week of gastric perfusion. Q PCR was used to detect the expression of propro TRH)mRNA in hypothalamus. The serum T3T4 levels were measured by electrochemiluminescence (ECL) at the 8th week of gastric perfusion. Q PCR and Western blot were used to detect the expression of NISN mRNA and protein in thyroid tissue. The level of T4 in Met group was higher than that in HT group (P 0.05). The results of H-E staining showed that the follicles in NC group varied in size after 8 weeks of gastric perfusion. A large number of colloid and absorption vacuoles were found. In HT group, thyroid follicles decreased, follicular epithelium became columnar, and vacuoles around follicles decreased. Follicular epithelial hyperplasia in Met group was slightly improved than that in HT group. Q PCR showed that the expression of pro TRH in hypothalamus and NIS mRNA in thyroid tissue in HT group was higher than that in NC group, while that in Met group was lower than that in HT group. The expression of NIS protein in Met group was lower than that in HT group (P 0.05). However, there was no significant difference in NIS expression between HT group and NC group (P 0.05). 12 weeks after gastric perfusion, there was no significant difference in the expression of NIS protein between HT group and NC group. There was no significant difference in NIS mRNA expression between HT group and Met group (P 0.05). The expression of NIS protein in Met group was higher than that in HT group P0.05.Conclusion Metformin can increase the level of thyroid hormone in hypothyroidism rats. In the short term, however, it was not caused by increased NIS expression in the thyroid gland.
【作者单位】：第二军医大学长征医院内分泌科;
【分类号】：R581.2
【正文快照】： [Acad J Sec Mil Med Univ,2017,38(5):589-594]在糖尿病患者中,甲状腺功能异常的发生率居高不下,其中以甲状腺功能减退(以下简称“甲减”)最为常见[1]。一项基于1 310例糖尿病患者的大型临床研究显示,甲状腺功能障碍的总体患病率为13.4%,其中包括8.8%的男性和16.8%的女性,明

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