VEGF rs3025039基因多态性及血浆水平，与强直性脊柱炎易感性的关联研究

发布时间：2018-02-10 12:38

本文关键词： 血管内皮生长因子血浆浓度多态性强直性脊柱炎　出处：《南京医科大学》2017年博士论文　论文类型：学位论文

【摘要】：目的:强直性脊柱炎是一种自身免疫性炎症性疾病,以腰痛,骶髂关节炎,和脊柱强直为临床特点。强直性脊柱炎的发病机制尚不清楚,与血管生成和遗传背景相关的各种生物标志物在强直性脊柱炎在发病过程中起重要作用。血管内皮生长因子是一种促进血管内皮细胞迁移和增殖的血管生成因子,具有增加血管通透性和介导炎症的作用。血管生成在多种风湿性疾病,包括类风湿关节炎,银屑病性关节炎,强直性脊柱炎,系统性硬化症中发挥作用,表明抑制血管生成可能是一个有用的治疗方法。血管内皮生长因子是最有力的促血管生成分子,并导致强直性脊柱炎的血管生成。我们假设,血管内皮生长因子多态性和血浆水平可能有助于强直性脊柱炎的易感性。方法:对强直性脊柱炎患者208例和412例住院其他患者对照组的病例对照研究,检验强直性脊柱炎风险性,VEGF基因型,中国人群中血管内皮生长因子的血浆水平之间的关联。使用基质辅助激光解吸电离飞行时间质谱进行基因分型。对其中120例患者和120名对照组患者,使用酶联免疫吸附测定法测定血浆VEGF浓度水平。结果:我们发现,血管内皮生长因子rs3025039 C/T基因多态性增加强直性脊柱炎的患病风险,在强直性脊柱炎患者组中,血管内皮生长因子的平均血浆浓度相比对照组显著较高。在强直性脊柱炎患者组中,男性血管内皮生长因子水平明显高于女性。分层后,rs3025039 CC基因型强直性脊柱炎患者的平均血浆浓度明显高于对照组。血浆VEGF水平与年龄、HLA-B27、CRP或骶髂关节评分,在患者组和对照组之间没有显著差异。结论:这项研究证据表明,有可能血管内皮生长因子rs3025039多态性,血管内皮生长因子血浆浓度和强直性脊柱炎患病风险之间存在联系。然而,我们的结果是从一个中等大小的样品,因此这是一个初步的结论。需在一个更大和更多样化的种族样本中验证,以便确认这些发现。
[Abstract]:Objective: ankylosing spondylitis is an autoimmune inflammatory disease characterized by lumbago sacroiliac arthritis and spinal ankylosis. Various biomarkers associated with angiogenesis and genetic background play an important role in the pathogenesis of ankylosing spondylitis. Vascular endothelial growth factor (VEGF) is an angiogenic factor that promotes the migration and proliferation of vascular endothelial cells. Angiogenesis plays a role in many rheumatoid diseases, including rheumatoid arthritis, psoriatic arthritis, ankylosing spondylitis, systemic sclerosis. This suggests that inhibition of angiogenesis may be a useful treatment. Vascular endothelial growth factor is the most potent angiogenic molecule and causes angiogenesis in ankylosing spondylitis. Vascular endothelial growth factor polymorphism and plasma levels may contribute to the susceptibility of ankylosing spondylitis. Methods: a case-control study was conducted in 208 patients with ankylosing spondylitis and 412 other hospitalized controls. To test the genotype of VEGF in the risk of ankylosing spondylitis, Association between plasma levels of vascular endothelial growth factor in Chinese population. Genotyping using matrix assisted laser desorption ionization time of flight mass spectrometry. 120 patients and 120 controls. Results: we found that the polymorphism of vascular endothelial growth factor (rs3025039) C / T gene increased the risk of ankylosing spondylitis in patients with ankylosing spondylitis. The average plasma concentration of vascular endothelial growth factor was significantly higher than that of the control group. After stratification, the mean plasma concentration of VEGF in male was significantly higher than that in female. The average plasma concentration in patients with ankylosing spondylitis genotype rs3025039CC was significantly higher than that in control group. The plasma VEGF level and age of HLA-B27C-CRP or sacroiliac joint score were significantly higher than those in control group. There was no significant difference between the patient group and the control group. Conclusion: the evidence in this study suggests the possibility of vascular endothelial growth factor rs3025039 polymorphism. There is a link between the plasma concentration of vascular endothelial growth factor and the risk of ankylosing spondylitis. So this is a preliminary conclusion. It needs to be tested in a larger and more diverse ethnic sample in order to confirm these findings.
【学位授予单位】：南京医科大学
【学位级别】：博士
【学位授予年份】：2017
【分类号】：R593.23

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