hUCMSCs移植提高围绝经期大鼠血清雌激素水平的持续时间及对卵巢P450arom表达的影响

发布时间：2018-02-10 15:50

本文关键词： hUCMSCs移植围绝经期雌激素细胞色素P芳香化酶　出处：《现代妇产科进展》2017年03期 　论文类型：期刊论文

【摘要】：目的:探讨人脐带间充质干细胞(h UCMSCs)移植提高围绝经期大鼠血清雌激素(E2)水平的持续时间及其对卵巢细胞色素P450芳香化酶(P450arom)表达的影响。方法:阴道脱落细胞涂片法筛选出30只SPF级12~14月龄的围绝经期SD大鼠,随机分为A、B组;同时筛选出15只SPF级3~5月龄具有生育能力的青年雌性SD大鼠,作为C组。B组:尾静脉移植浓度为1×10~6cells/ml的h UCMSCs单细胞悬液1ml,隔日1次,共两次;A组:与B组在相同时间相同方式移植等量PBS液;C组:不予处理。于移植前所有大鼠经眼眶采血检测血清E2水平,并于末次移植后14d、21d、28d每组随机选取5只大鼠采血并取双侧卵巢组织,ELISA法检测血清E_2水平、免疫组化及Western blot法检测卵巢P450arom表达水平。结果:末次移植后14d、21d、28d,B组大鼠血清E_2水平、卵巢P450arom表达与移植前及A组相比均明显升高,差异有统计学意义(P0.05),B组3个时间段的E_2水平、P450arom表达无明显差异(P0.05)。结论:h UCMSCs移植提高围绝经期大鼠血清E_2水平可至少持续至移植后28d;h UCMSCs移植可促进围绝经期大鼠卵巢细胞色素P450芳香化酶表达,是其提高E_2水平的可能途径。
[Abstract]:Objective: to investigate the effect of human umbilical cord mesenchymal stem cells (UCMSCs) transplantation on the expression of cytochrome P450 aromatase (P450 aromase) in the serum of postmenopausal rats. Methods: vaginal exfoliated cells were coated with vaginal exfoliated cells. Thirty postmenopausal SD rats of SPF grade 12 and 14 months of age were screened by slice method. At the same time, 15 young female Sprague-Dawley (SD) rats of SPF grade 3 and 5 months old were selected as group C. Group B: single cell suspension of h UCMSCs at the concentration of 1 脳 10 6 cells / ml, 1 ml every other day. Group A: group C received the same amount of PBS solution at the same time and the same time as group B: no treatment. Before transplantation, all rats were collected blood from orbit to detect serum E2 level. The serum E2 level was detected by Elisa in bilateral ovarian tissues, P450 arom expression in ovary by immunohistochemical and Western blot methods. Results: the serum E2 level of rats in group B was measured by Elisa, and the serum E2 level was detected by immunohistochemical staining and Western blot method on the 14th day, 21d, 28d, 28d after the last transplantation. The expression of P450 arom in ovary was significantly higher than that in group A and before transplantation. There was no significant difference in the expression of P450arom in three periods of P0.05A group. Conclusion UCMSCs transplantation can increase serum E2 level in perimenopausal rats at least until 28 days after transplantation. UCMSCs transplantation can promote peri-menopausal oocytes. Nest cytochrome P450 aromatase expression, It is a possible way to improve the level of E _ 2.
【作者单位】：广东医科大学教学医院广东省第二人民医院;
【基金】：广东省医学科学技术研究基金(No:A2016497)
【分类号】：R711.75

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