碘过量不同处理时间对Fischer大鼠甲状腺细胞线粒体过氧化损伤的影响

发布时间：2018-02-14 03:33

  本文关键词： 碘过量 Fischer大鼠 甲状腺细胞线粒体 过氧化损伤　出处：《中国老年学杂志》2017年18期 　论文类型：期刊论文

【摘要】：目的探讨碘过量不同处理时间对Fischer大鼠甲状腺细胞线粒体过氧化损伤的影响。方法采用含0(对照组)、0.1 mmol/L碘化钾的Coons F12培养基培养Fischer大鼠甲状腺细胞2、6 h、1 d,对小鼠抗大鼠细胞色素C蛋白、死亡细胞百分比、线粒体超氧化物生成、乳酸脱氢酶活力进行检测分析研究。结果 0.1 mmol/L碘化钾组处理2 h、6 h、1 d时,小鼠抗大鼠细胞色素C强阳性细胞率远高于对照组(P0.05);Fischer大鼠甲状腺细胞线粒体超氧化物生成明显高于对照组(P0.05),且处理2 h时增长幅度最大;随着0.1 mmol/L碘化钾处理时间的增加,培养液上清中乳酸脱氢酶活力程序持续增加趋势,处理2、6 h、1 d时乳酸脱氢酶活力明显高于对照组(P0.05)。结论碘过量处理2 h可能会导致Fischer大鼠甲状腺细胞线粒体发生过氧化损伤,而处理1 d则极有可能导致细胞凋亡。
[Abstract]:Objective to investigate the effect of iodine excess treatment on the mitochondrial peroxidation injury of thyroid cells in Fischer rats. Methods the thyroid cells of Fischer rats were cultured on Coons F12 medium containing 0 (control group) 0.1 mmol/L potassium iodide for 2 hours for 1 day. Anti rat cytochrome C protein, The percentage of dead cells, the formation of superoxide in mitochondria and the activity of lactate dehydrogenase were detected and analyzed. Results the results showed that the 0.1 mmol/L potassium iodide group was treated with potassium iodide for 2 h and 6 h for 1 day. The rate of anti-cytochrome C strong positive cells in mice was much higher than that in control group P0.05 Fischer rats, and the production of mitochondrial superoxide in thyroid cells was significantly higher than that in control group P0.05, and the maximal increase was observed at 2 h after treatment, with the increase of 0.1 mmol/L potassium iodide treatment time. The activity of lactate dehydrogenase in the supernatant of culture medium continued to increase, and the activity of lactate dehydrogenase in the supernatant was significantly higher than that in the control group at 2 h after treatment for 6 h. Conclusion iodine excess treatment for 2 h may cause peroxidation damage to the mitochondria of thyroid cells in Fischer rats. On the other hand, the treatment for 1 day is likely to lead to apoptosis.
【作者单位】： 深圳市坪山新区妇幼保健院药剂科;
【基金】：广东省卫生厅资助项目(No.WSTJJ20121204) 深圳市坪山新区医疗卫生发展孵化资助资金课题(No.201333)
【分类号】：R581
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