苦精对哮喘小鼠气道中钙结合蛋白S100A9及气道黏液分泌的影响

发布时间：2018-02-14 06:21

本文关键词： 苯甲地钠铵(苦精) 哮喘苦味受体钙结合蛋白SA 气道黏液　出处：《医学研究生学报》2017年02期 　论文类型：期刊论文

【摘要】：目的研究发现在人体肺部存在苦味受体,目前关于苦味受体激动剂与气道黏液分泌的研究报道较少。探讨肺部苦味受体激动剂苯甲地钠铵(苦精)对哮喘小鼠气道中钙结合蛋白S100A9水平和气道中黏液分泌的影响。方法将健康清洁型雄性BALB/c小鼠按随机数字表分成5组:正常对照组,慢性哮喘组,苦精干预1、2、3周组,每组8只。除正常对照组外,其余组小鼠于第1天和第15天分别腹腔注射0.2 m L氢氧化铝混悬液[含100μg OVA,1 mg Al(OH)3)]致敏。苦精干预1、2、3周组小鼠分别从第10、9、8周起在激发的基础上吸入800μg苦精1、2、3周。ELISA法及免疫组化法检测肺泡灌洗液(BALF)上清、肺组织中S100A9蛋白水平,糖原PAS染色及实时定量PCR显示气道杯状细胞数量、黏液性物质及黏蛋白MUC5AC的含量。结果与正常对照组BALF上清中S100A9水平[(6.19±0.61)ng/m L]比较,慢性哮喘组以及苦精干预1、2周组[(11.89±0.77)、(10.69±0.79)、(9.49±0.99)ng/m L]明显升高(P0.05);苦精干预1、2、3周组较慢性哮喘组降低(P0.05)。与正常对照组比较,免疫组化染色显示慢性哮喘组气道上皮细胞中S100A9高表达,苦精干预1、2、3周后,气道上皮细胞中S100A9表达随苦精干预时间增加而减少。糖原PAS染色显示,与正常对照组比较,慢性哮喘组气道上皮杯状细胞增生,黏液过度分泌(P0.01)。与慢性哮喘组比较,苦精干预1、2、3周组杯状细胞数量、黏液分泌量随苦精干预时间的增加而减少。与正常对照组比较,慢性哮喘组以及苦精干预1、2周组肺组织中黏蛋白MUC5AC mRNA表达增高,差异有统计学意义(P0.05),与慢性哮喘组比较,苦精干预后,MUC5AC的mRNA表达降低,并随苦精干预时间的增加而降低,差异有统计学意义(P0.05)。结论苦精下调小鼠杯状细胞的黏液分泌可能与上皮细胞钙结合蛋白S100A9有关。
[Abstract]:Objective to study the existence of bitter receptors in human lungs. There are few reports about bitter receptor agonists and airway mucus secretion. To explore the effects of pulmonary bitter receptor agonist benzodimethylammonium (bitter essence) on the levels of calcium binding protein S100A9 in the airway and mucus secretion in the airway of asthmatic mice. Methods healthy and clean male BALB/c mice were randomly divided into 5 groups: normal control group. In the chronic asthma group, 8 rats in each group were treated with bitter extract for 3 weeks, except for the normal control group. The mice in the other group were sensitized by intraperitoneal injection of 0.2 mL aluminum hydroxide suspension (containing 100 渭 g OVAX 1 mg Albumin 3) on day 1 and day 15, respectively. And immunohistochemical method was used to detect the supernatant of alveolar lavage fluid (BALF). The levels of S100A9 protein in lung tissue, glycogen PAS staining and real-time quantitative PCR showed the number of goblet cells, mucin and mucin MUC5AC in the airway. The results were compared with those in the normal control group (6.19 卤0.61g / mL). The expression of S100A9 in airway epithelial cells of chronic asthma group was significantly higher than that of chronic asthma group after 1 ~ 2 weeks of treatment [11.89 卤0.77 ~ 10.69 卤0.79 ng / mL], and the expression of S100A9 in airway epithelial cells of chronic asthma group was significantly higher than that of chronic asthma group at 3 weeks after treatment with bitter spermatine for 1 ~ 2 ~ 2 weeks. Compared with normal control group, the expression of S100A9 in airway epithelial cells in chronic asthma group was significantly higher than that in control group, and the expression of S100A9 in airway epithelial cells in chronic asthma group was significantly higher than that in control group. The expression of S100A9 in airway epithelial cells decreased with the increase of the time of intervention. Glycogen PAS staining showed that, compared with the normal control group, the airway epithelial goblet cells proliferated in chronic asthma group. Compared with the chronic asthma group, the number of goblet cells in the group treated with bitter spermatine for 3 weeks was decreased with the increase of the time of the intervention. Compared with the normal control group, the number of goblet cells decreased with the increase of the time of the treatment. The expression of mucin MUC5AC mRNA in the lung tissue of chronic asthma group and the group treated with bitter essence for 1 to 2 weeks was increased, the difference was statistically significant (P 0.05). Compared with the group of chronic asthma, the expression of MUC5AC mRNA in bitter dry prognosis was lower than that in chronic asthma group, and the expression of MUC5AC decreased with the increase of the time of intervention. Conclusion the down-regulation of mouse goblet cell mucus secretion may be related to the calcium binding protein S100A9.
【作者单位】：潍坊医学院临床医学院;潍坊医学院附属医院呼吸内科;
【分类号】：R562.25

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