热射病大鼠死亡危险因素及心肌损伤的实验研究

发布时间：2018-02-15 01:43

  本文关键词： 热射病 动物模型 心肌损伤 氧化应激 死亡率　出处：《中国现代医学杂志》2017年14期 　论文类型：期刊论文

【摘要】：目的复制热射病(HS)大鼠模型,探讨HS大鼠死亡危险因素及心肌损伤情况。方法雄性无特定病原体级SD大鼠48只,随机分为对照组、HS组、维生素E组(HS维生素E处理)、缬沙坦组(HS缬沙坦处理),每组12只;除对照组外,余组给予40℃、(65±1)%湿热打击,达复制模型标准后终止;心肌苏木精-伊红染色法(HE)染色及透射电镜观察病理变化。结果对照组体重变化率与其余3组比较,差异有统计学意义(P0.05),HS组血清乙酰胆碱(Ach)含量为(9.958±4.283)μg/ml,对照组为(15.734±4.987)μg/ml,两组比较,差异有统计学意义(P0.05);HE染色和透射电镜结果显示,HS大鼠存在心肌损伤,且维生素E组、缬沙坦组大鼠心肌损伤程度较HS组减轻。结论死亡事件主要发生在发病后24 h内,且低体温和较长打击时间是大鼠死亡的主要危险因素,但相对较高的体重变化率是大鼠存活的保护因素;HS大鼠存在心肌损伤,其潜在机制可能是氧化应激和血管紧张素Ⅱ诱导的心肌细胞凋亡。
[Abstract]:Objective to study the risk factors of death and myocardial injury in HS rats. Methods 48 male SD rats with no specific pathogen were randomly divided into control group and HS group. Vitamin E group treated with HS vitamin E and valsartan group treated with HS valsartan with 12 rats in each group. Results compared with the other three groups, the serum acetylcholine acetylcholine (ache) content in the control group was 9.958 卤4.283 渭 g / ml, and the control group was 15.734 卤4.987 渭 g / ml. The results of HE staining and transmission electron microscope showed that there was myocardial injury in HS rats, and the degree of myocardial injury in vitamin E group and valsartan group was less than that in HS group. Hypothermia and long hit time were the main risk factors of rat death, but the relatively high rate of body weight change was the protective factor of survival in HS rats. The underlying mechanism may be oxidative stress and angiotensin 鈪，

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