海马内不同亚型雌激素受体α及相关信号分子与糖尿病小鼠空间认知障碍的相关性

发布时间：2018-02-20 07:23

本文关键词： 空间学习记忆雌激素受体α 糖尿病认知障碍　出处：《生理学报》2017年03期 　论文类型：期刊论文

【摘要】：本研究旨在探讨海马内不同亚型雌激素受体α(estrogen receptorα,ERα)及相关信号分子与糖尿病引起的空间认知障碍的相关性。腹腔注射四氧嘧啶建立1型糖尿病小鼠模型,并采用Morris水迷宫方法检测模型组小鼠是否存在空间认知障碍;然后用Western blot比较模型组和正常对照组小鼠海马内ERα不同亚型ER-α36和ER-α66的表达,同时检测窖蛋白-1(caveolin-1)、PKCα、cAMP反应元件结合蛋白2(cAMP-response element binding protein 2,CREB2)和突触素(synaptophysin,Syn)的表达变化。结果显示,相对对照组,糖尿病模型组小鼠空间训练第3天和第5天的逃避潜伏期显著延长(P0.05),撤去平台后游泳路程增加;模型组小鼠海马caveolin-1、PKCα表达量显著降低(P0.05),ER-α66蛋白表达水平没有明显变化,而ER-α36和CREB2的表达量显著升高(P0.05)。以上结果提示,海马内ER-α36及相关信号分子的异常表达对糖尿病小鼠空间认知障碍形成可能具有重要的作用。
[Abstract]:The purpose of this study was to investigate the relationship between estrogen receptor 伪 estrogen receptor 伪 er 伪 and related signal molecules in hippocampus and spatial cognitive impairment induced by diabetes mellitus. Type 1 diabetic mice were induced by intraperitoneal injection of alloxan. Morris water maze method was used to detect whether there was spatial cognitive impairment in model group, and then Western blot was used to compare the expression of ER- 伪 36 and ER- 伪 66 in hippocampus between model group and normal control group. At the same time, the expression of cellar protein-1 caveolin-1 (PKC 伪) and synaptophysinine synthase (CREB2) were detected. The results showed that the expression of cAMP-response element binding protein 2 (CREB2) and synaptophysinsinine synthase (synaptopsinsin) in the control group was higher than that in the control group. The escape latency on the 3rd and 5th day of spatial training in diabetic model group was significantly prolonged and the swimming distance increased after removing the platform, but the expression of caveolin-1 PKC 伪 in hippocampus of model group did not change significantly. These results suggest that the abnormal expression of ER- 伪 36 and related signal molecules may play an important role in the formation of spatial cognitive impairment in diabetic mice.
【作者单位】：辽宁师范大学生命科学学院;中国人民解放军第二一零医院;大连市中心医院神经内科;卫生部细胞移植重点实验室临床中心;
【基金】：supported by the National Natural Science Foundation of China(No.30970353) the Science and Technology Project of Liaoning Province,China(No.201501522)
【分类号】：R587.2;R749.2

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