经尿道灌注干细胞白血病重组基因慢病毒对糖尿病膀胱病变豚鼠膀胱功能的影响

发布时间：2018-02-20 19:29

本文关键词： 糖尿病膀胱病变慢病毒干细胞白血病基因膀胱功能 Cajal间质细胞豚鼠　出处：《山东医药》2017年30期 　论文类型：期刊论文

【摘要】：目的探讨经尿道灌注干细胞白血病重组(SCL)基因慢病毒对糖尿病膀胱病变(DCP)豚鼠膀胱功能的改善作用。方法采用单次腹腔注射链脲佐菌素(STZ)法建立豚鼠糖尿病模型,培养12周后建立DCP模型。选取DCP豚鼠20只,随机分为模型组、SCL组各10只。取未造模的10只作为对照组。各组麻醉后经尿道插入自制尿管,排空尿液,对照组和模型组均经尿管灌注PBS液0.2 mL,SCL组经尿管灌注最佳感染复数(MOI)的SCL基因慢病毒0.2 mL(2×10~7IU)。灌注后结扎尿管,保留膀胱灌注液2 h。灌注后28 d时用代谢笼收集三组24 h尿量;行尿动力学检测,于膀胱排尿后即刻测残余尿量、最大膀胱容量、排尿期最大逼尿肌压、单次排尿周期灌注量、膀胱顺应性。切除完整膀胱,排尽尿液后称量膀胱湿重。结果与对照组相比,模型组和SCL组24 h尿量、膀胱湿重、最大膀胱容量、残余尿量均增加,最大逼尿肌压、膀胱顺应性均降低(P均0.05);与模型组相比,SCL组24 h尿量、膀胱湿重、最大膀胱容量、残余尿量均降低,最大逼尿肌压、膀胱顺应性均增加(P均0.05)。结论经尿道灌注SCL慢病毒能够显著改善DCP豚鼠的膀胱功能,为治疗DCP提供了新的途径与方法。
[Abstract]:Objective to evaluate transurethral perfusion of stem cell leukemia (SCL) gene recombinant lentiviral vector on diabetic cystopathy (DCP) to improve the effect of bladder function in guinea pigs. Methods a single intraperitoneal injection of streptozotocin (STZ) method to establish the guinea pig model of diabetes, to establish the DCP model after 12 weeks. 20 DCP guinea pigs were selected. Were randomly divided into model group, SCL group with 10 rats in each group. 10 rats as the control group without modeling. Each group after anesthesia transurethral insertion of self-made catheter, emptying urine, control group and model group were treated by catheter perfusion with PBS solution for 0.2 mL, SCL group after the catheter perfusion optimal multiplicity of infection (MOI) of the SCL gene in slow virus 0.2 mL (2 * 10~7IU). After ligation of perfusion catheter, bladder perfusion fluid retention 2 h. After Reperfusion 28 d using metabolic cages collected from three groups of 24 h urine volume; urodynamic testing, in the immediate post test of bladder residual urine volume, maximum bladder capacity, maximum detrusor pressure during voiding, single urination Periodic perfusion of bladder compliance. Complete resection of the bladder, as urine after weighing the weight of bladder. Results compared with the control group, model group and SCL group, 24 h urine volume, bladder wet weight, maximum bladder capacity, residual urine volume increased, maximum detrusor pressure, bladder compliance decreased (P < 0.05); compared with the model group, SCL group, 24 h urine volume, bladder wet weight, maximum bladder capacity, residual urine volume decreased, maximum detrusor pressure, bladder compliance increased (P < 0.05). Conclusion transurethral perfusion SCL lentivirus can significantly improve bladder function of guinea pigs with DCP, provides a way and method the new treatment for DCP.

【作者单位】：石河子大学医学院第一附属医院;
【基金】：国家自然科学基金资助项目(81360120)
【分类号】：R587.2

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