脂多糖协同MSU诱导THP1分泌IL-1β和IL-18

发布时间：2018-02-24 15:43

  本文关键词： 细胞免疫学 尿酸钠结晶 脂多糖 痛风 炎症体 白细胞介素-β 白细胞介素-　出处：《深圳大学学报(理工版)》2016年06期 　论文类型：期刊论文

【摘要】：通过合成尿酸钠结晶(monosodium urate crystals,MSU)分析其对人外周血单核细胞系THP1的促炎作用,分别以0、1.0、10.0和100.0μmol/L的MSU刺激THP1细胞4 h,检测不同浓度的MSU对炎症体组分Nod样受体蛋白3(Nod-like receptor protein 3,NLRP3)、半胱氨酸天冬氨酸特异性蛋白水解酶(caspase-1)及促炎因子白细胞介素-1β(interleukin-1β,IL-1β)和IL-18的信使核糖核酸(messenger ribonucleic acid,mRNA)表达的影响.为研究toll样受体4(toll like receptor 4,TLR4)的配体脂多糖的作用,用1.0 ng/m L的脂多糖(lipopolysaccharide,LPS)预刺激THP1细胞3 h,再加100.0μmol/m L的MSU刺激细胞,通过实时荧光定量聚合酶链式反应及酶联免疫吸附试验,检测LPS对MSU诱导的促炎因子IL-1β和IL-18的mRNA及蛋白水平.研究发现,在THP1细胞中,100.0μmol/L的MSU即可显著激活炎症体的mRNA,但其对促炎因子IL-1β及IL-18的mRNA表达的诱导作用却不明显,加入1.0 ng/m L LPS预刺激3 h后,MSU诱导的THP1细胞IL-1β和IL-18的表达量显著增加.证明脂多糖对MSU诱导THP1细胞产生IL-1β和IL-18有协同作用.
[Abstract]:The effect of sodium uric acid on the inflammation of human peripheral blood monocyte cell line THP1 was analyzed by synthesizing monosodium urate crystal MSU. THP1 cells were stimulated with 0 0 渭 mol/L and 100 0 渭 mol/L MSU for 4 h, respectively. The effects of different concentrations of MSU on Nod like receptor protein 3nd-like receptor protein 3nLRP3, caspase-1 and interleukin-1 尾 interleukin-1 尾 interleukin 1 (IL-1 尾) and IL-18 were detected. In order to study the effect of ligand lipopolysaccharide (LPS) on the expression of toll like receptor 4toll like receptor 4 (TLR4), THP1 cells were prestimulated with 1. 0 ng/m / L lipopolysaccharide for 3 h and then stimulated with MSU of 100 渭 mol/m L for 3 h. Real-time quantitative polymerase chain reaction and enzyme-linked immunosorbent assay (Elisa) were used. The levels of mRNA and protein of IL-1 尾 and IL-18 induced by MSU were detected by LPS. It was found that MSU of 100.0 渭 mol/L could significantly activate the expression of inflammatory mRNAs in THP1 cells, but its effect on the expression of IL-1 尾 and IL-18 in THP1 cells was not obvious. After pretreatment with 1.0 ng/m L LPS for 3 h, the expression of IL-1 尾 and IL-18 in THP1 cells induced by MSU was significantly increased, which indicated that LPS had synergistic effect on MSU induced IL-1 尾 and IL-18 production in THP1 cells.
【作者单位】： 深圳大学医学部;深圳市人民医院暨南大学第二临床医学院风湿免疫科;
【基金】：中国博士后科学基金资助项目(2015M572370) 深圳市科技计划资助项目(JCYJ20130402092657775)~~
【分类号】：R589.7
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本文编号：1530822