Ki-67在肥胖PCOS大鼠模型卵巢颗粒细胞中的表达研究

发布时间：2018-02-28 09:22

本文关键词： Ki- 肥胖PCOS大鼠模型颗粒细胞表达研究　出处：《辽宁中医杂志》2016年06期 　论文类型：期刊论文

【摘要】：目的:探讨子宫内膜生存素Ki-67在肥胖多囊卵巢综合(PCOS)大鼠模型卵巢颗粒细胞中的表达。方法:分别在来曲唑(Letrozole)和脱氢表雄酮(DHEA)造模基础上加用高脂乳剂,建立肥胖PCOS动物模型,结合血清性激素、大鼠处死时体重增加幅度及脏器指数改变进行模型验证,选用免疫组织化学法检测大鼠模型卵巢组织卵泡颗粒细胞中Ki-67的表达。结果:模型建立成功。Letrozole模型组和DHEA模型组Ki-67积分光密度总和、平均灰度均值显著低于空白对照组(P0.05),此外,Letrozole肥胖模型组和DHEA肥胖模型组Ki-67积分光密度总和、平均灰度均值极显著低于空白对照组和高脂模型组(P0.01);Letrozole肥胖模型组Ki-67平均灰度均值显著低于Letrozole模型组(P0.05),DHEA肥胖模型组Ki-67平均灰度均值显著低于DHEA模型组(P0.05),差异有统计学意义。结论:Ki-67在PCOS大鼠模型卵巢的卵泡颗粒细胞中表达降低可能是PCOS发生发展的重要原因,且Ki-67在肥胖PCOS大鼠模型卵巢卵泡颗粒细胞中表达降低更明显。
[Abstract]:Objective: to investigate the expression of endometrial survivin Ki-67 in ovarian granulosa cells of obese rat model of polycystic ovary synthesis. Methods: the obese PCOS animal model was established by adding high fat emulsion on the basis of Letrozole and Dehydroepiandrosterone respectively. Combined with serum sex hormones, the changes of body weight gain and organ index of the rats at the time of death were used to verify the model. Immunohistochemical method was used to detect the expression of Ki-67 in follicular granulosa cells of rat ovarian tissue. Results: the total integrated optical density of Ki-67 in the model group and DHEA model group was successfully established. The average gray level was significantly lower than that of the blank control group (P 0.05), in addition, the total Ki-67 integral optical density in the Letrozole obesity model group and the DHEA obesity model group was significantly lower than that in the control group. The average gray level of Ki-67 in the obese model group was significantly lower than that in the Letrozole model group and that in the control group and the high-fat model group. The average gray value of Ki-67 in the obese model group was significantly lower than that in the DHEA model group. The difference was statistically significant. Conclusion the decrease of the expression of + Ki-67 in follicular granulosa cells of ovary of PCOS rat model may be an important reason for the occurrence and development of PCOS. The expression of Ki-67 in ovarian follicular granulosa cells was significantly decreased in obese PCOS rats.
【作者单位】：成都中医药大学;
【基金】：国家自然科学基金(81470199) 成都中医药大学科技发展基金(ZRMS201307)
【分类号】：R711.75;R-332

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