维生素D对绝经后骨质疏松症基质Gla蛋白表达的影响

发布时间：2018-03-04 08:23

本文选题：维生素D　切入点：基质Gla蛋白　出处：《南昌大学》2015年硕士论文　论文类型：学位论文

【摘要】：目的:1、观察绝经后骨质疏松患者血清基质Gla蛋白(MGP)与骨密度、血清MGP与25(OH)D3的关系;2、观察1,25(OH)2D3对人骨肉瘤细胞MG63的Wnt信号通路及MGP表达的影响,探讨1,25(OH)2D3在绝经后骨质疏松症防治作用中可能的新机制。方法:1、研究对象的纳入:2013年4月到2015年2月南昌大学第二附属医院门诊体检及住院的绝经后女性125人,按骨密度可分为骨密度正常组、低骨量组、骨质疏松组。测定血清MGP、25(OH)D3浓度,将绝经后骨质疏松症患者按25(OH)D3水平分为充足组、不足组、缺乏组。2、分别使用1,25(OH)2D3 10-8mol/L、DKK-1(Dickkopf-1)200 ng/ml、1,25(OH)2D3 10-8 mol/L+DKK-1 200ng/ml干预人骨肉瘤细胞MG63细胞株48小时,1%BSA(bovine serum albumin,牛血清白蛋白)+无酚红MEM培养作为空白对照组,使用实时荧光定量RT-PCR及western-blot技术测定Wnt/β-catenin信号通路中相关因子β-catenin、Runx2、LRP5及MGP基因及蛋白表达的情况。结果:1、绝经后骨质疏松症患者低骨量组、骨质疏松组血清MGP浓度明显低于正常组,差异有统计学意义(P0.05),且血清MGP与骨密度呈正相关。2、绝经后骨质疏松症患者的25(OH)D3缺乏组、不足组的血清MGP明显低于充足组,差异有统计学意义(P0.05)且血清MGP与25(OH)D3呈正相关。3、1,25(OH)2D3上调MG63细胞中Wnt/β-catenin信号通路中相关因子β-catenin、Runx2、LRP5时,MGP的表达也增加。DKK-1抑制Wnt/β-catenin信号通路的同时下调了MGP的表达,差异均有统计学意义(P0.05)。结论:1、绝经后骨质疏松症患者血清MGP与骨密度呈正相关。2、绝经后骨质疏松症患者血清MGP浓度与25(OH)D3呈正相关。3、1,25(OH)2D3有可能通过Wnt/β-catenin信号通路影响MGP的表达。
[Abstract]:Objective: To observe 1 postmenopausal osteoporosis serum matrix Gla protein (MGP) and bone mineral density, serum MGP and 25 (OH) D3; 2, 1,25 (OH) to observe the influence of 2D3 expression on human osteosarcoma cell line MG63 Wnt pathway and MGP, on 1,25 (OH) 2D3 in menopausal women after the osteoporosis prevention and treatment may be a new mechanism. Methods: 1, included in the study: from April 2013 to February 2015 in the Second Affiliated Hospital of Nanchang University medical outpatient and inpatient postmenopausal women 125 people, according to the bone density, bone mineral density can be divided into normal group, osteopenia group, osteoporosis group. Serum MGP, 25 (OH) D3 concentration, the postmenopausal osteoporosis patients by 25 (OH) D3 levels were divided into deficiency group, lack of adequate group,.2 group, respectively, using 1,25 (OH) 2D3 10-8mol/L DKK-1 (Dickkopf-1) 200 ng/ml, 1,25 (OH) 2D3 10-8 mol/L+DKK-1 200ng/ml intervention in human osteosarcoma cell line MG63 cells 48 hours, 1%BSA (bovine Serum albumin, bovine serum albumin) + phenol red free MEM medium as the blank control group, real-time fluorescence quantitative RT-PCR and Western-blot determination of related factor beta Wnt/ beta -catenin signaling pathway in -catenin, Runx2, LRP5 and MGP gene and protein expression. Results: 1 postmenopausal osteoporosis patients with low bone mass group, osteoporosis osteoporosis group serum MGP concentration was significantly lower than the normal group, the difference was statistically significant (P0.05), and the serum MGP was positively correlated with bone mineral density.2, the patients with osteoporosis after menopause 25 (OH) D3 deficient group, serum MGP deficiency group was significantly lower than that in sufficient group, the difference was statistically significant (P0.05) and the serum MGP and 25 (OH) D3 was positively correlated with.3,1,25 (OH) related factor beta Wnt/ beta -catenin signaling pathway in -catenin cells, 2D3 upregulation of MG63 Runx2, LRP5, MGP also increased expression of.DKK-1 inhibits Wnt/ beta -catenin signaling pathway and down-regulation of MGP The expression, the differences were statistically significant (P0.05). Conclusion: 1 postmenopausal osteoporosis patients serum MGP was positively correlated with bone mineral density.2, postmenopausal osteoporosis in patients with serum MGP concentration (OH) and 25 D3 (OH).3,1,25 was positively related to 2D3 through Wnt/ beta -catenin signaling pathway affect the expression of MGP.

【学位授予单位】：南昌大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R580

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