糖尿病难愈性创面大鼠模型的建立

发布时间：2018-03-05 10:13

本文选题：糖尿病　切入点：溃疡　出处：《山东医药》2016年14期 　论文类型：期刊论文

【摘要】：目的建立一种糖尿病难愈性创面大鼠模型。方法将34只雌性Wistar大鼠随机分为对照组和DM组各17只,适应性饲养1周后分别给予常规饲料及高脂高糖饲料喂养4周;DM组腹腔注射小剂量链脲佐菌素,对照组腹腔注射等量柠檬酸-柠檬酸钠缓冲液,4周后于两组大鼠背部制作直径约2.5 cm的圆形创面;创面造模后第3、7、14天,观察两组大鼠创面愈合情况并计算创面愈合率,HE染色法观察创面组织病理变化。结果 DM组创面愈合率分别为5.21%±0.94%、31.70%±1.21%、62.20%±1.37%,对照组分别为20.87%±1.39%、49.99%±1.58%、91.60%±1.58%,两组同时点比较P均0.05。与对照组相比,其创面组织中含有较少的炎性细胞、纤维母细胞及新生毛细血管。结论高脂高糖饲料饲养联合小剂量STZ注射制作2型糖尿病模型,维持高血糖4周后采用外科方法制作溃疡模型,可成功建立糖尿病难愈性创面模型。
[Abstract]:Objective to establish a diabetic rat model of refractory wound. Methods 34 female Wistar rats were randomly divided into control group (n = 17) and DM group (n = 17). After one week of adaptive feeding, small doses of streptozotocin were injected into the abdominal cavity of DM group, which were fed with conventional diet and high fat and high sugar diet for 4 weeks, respectively. The rats in the control group were treated with citric acid-sodium citrate buffer solution for 4 weeks and then made a circular wound about 2.5 cm in diameter on the back of the two groups. Results in DM group, the wound healing rate was 5.21% 卤0.940.The wound healing rate in DM group was 31.70% 卤1.21 + 0.21% 卤1.370.The wound healing rate in DM group was 20.87% 卤1.399.99% 卤1.58% 卤1.58%, respectively, compared with that in control group (P < 0.05). There were less inflammatory cells, fibroblasts and new capillaries in the wound tissue. Conclusion the model of type 2 diabetes was induced by high fat and high glucose diet and low dose STZ injection. After maintaining hyperglycemia for 4 weeks, the model of diabetic refractory wound can be successfully established by surgical method.
【作者单位】：山东中医药大学;山东中医药大学附属医院;
【基金】：山东省自然科学基金资助项目(ZR2012HL14) 山东省济南市青年科技明星计划(20120145)
【分类号】：R587.2;R-332

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