去卵巢对骨质疏松大鼠皮肤衰老的影响

发布时间：2018-03-06 07:21

本文选题：大鼠　切入点：去卵巢　出处：《中国实验动物学报》2017年03期 　论文类型：期刊论文

【摘要】：目的用去卵巢大鼠构建的骨质疏松症模型是研究绝经后骨质疏松疾病的最适模型,然而大鼠卵巢去除后对皮肤的影响如何,这方面的研究甚少。本研究旨在观察去卵巢对大鼠皮肤衰老指标的影响。方法 3月龄健康SD雌性大鼠20只,随机分为假手术组(Sham组)和去卵巢组(OVX组),卵巢去除后3个月,测定血清雌二醇,以及皮肤组织中与衰老相关的生化指标如过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)活性以及丙二醛(MDA)、羟脯氨酸(HYP)、脂褐质(LF)含量,并观察皮肤组织病理学变化,用计算机图像分析系统定量分析。结果 OVX组大鼠血清雌二醇水平显著降低;组织病理学观察显示OVX组表皮、真皮厚度较Sham组显著变薄(P0.01),皮肤胶原纤维减少,排列疏松;与Sham组相比,OVX组大鼠皮肤CAT、GSH-Px和SOD活性显著降低(P0.01),MDA含量较Sham组明显增加(P0.01),皮肤组织中HYP含量明显减少,LF含量显著增多(P0.01)。结论 3月龄大鼠卵巢去除后3个月可导致皮肤衰老,可能是研究绝经后皮肤衰老的适宜模型。
[Abstract]:Objective Osteoporosis model in ovariectomized rats is the best model for studying postmenopausal osteoporosis. The purpose of this study was to observe the effect of ovariectomy on skin senescence in rats. Methods Twenty healthy female SD rats aged 3 months were randomly divided into sham-operated group (Sham group) and ovariectomized group (OVX group). Serum estradiol, and the activities of catalase CATX, glutathione peroxidase (GSH-PxX), superoxide dismutase (SOD) and the contents of malondialdehyde (MDA), hydroxyproline (HYP), lipofuscin (LFLF) in skin tissue were measured. The histopathological changes of skin were observed and quantitatively analyzed by computer image analysis system. Results the serum estradiol level in OVX group was significantly decreased, and the histopathological observation showed that the epidermis of OVX group, The thickness of dermis was significantly thinner than that of Sham group (P 0.01), the collagen fibers of skin decreased and the arrangement was loose. Compared with Sham group, the activity of GSH-Px and SOD in skin of OVX group was significantly lower than that of Sham group. Compared with Sham group, the content of HYP in skin tissue was significantly decreased, and the content of LF was significantly increased. Conclusion 3 months after the removal of ovary of 3 months old rats, skin senescence can be induced. It may be a suitable model for studying postmenopausal skin aging.
【作者单位】：陕西理工大学维生素D生理与应用研究所;
【基金】：陕西省科技创新团队项目(2012KCT-29) 陕西省科协青年人才托举项目(20160128) 秦巴山区生物资源综合开发协同创新中心培育项目(QBXT-Z(P)-15-18) 陕西理工大学博士后项目(SLGBH16-03)
【分类号】：R-332;R580

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