十二指肠空肠旁路术对ZDF大鼠BP肠袢部位炎症状态的改善作用及其机制

发布时间：2018-03-07 09:49

本文选题：十二指肠空肠旁路术　切入点：型糖尿病　出处：《吉林大学学报(医学版)》2017年06期 　论文类型：期刊论文

【摘要】：目的:探讨十二指肠空肠旁路术(DJB)对肥胖型2型糖尿病(T2DM)大鼠血糖稳态、胰岛素抵抗及炎症的改善作用,阐明其可能机制。方法:20只ZDF大鼠随机分为DJB组和假手术组,每组10只。每组存活8只大鼠。分别在手术前1周和手术后2、4、6周采用罗氏血糖仪测定2组大鼠空腹血糖(FBG)水平,ELISA法检测大鼠血清胰岛素水平,计算大鼠胰岛素敏感指数(HOMA-ISI)和胰岛素抵抗指数(HOMA-IR)。手术后6周处死大鼠,HE染色观察大鼠BP肠袢炎性细胞形态表现,免疫组织学法观察大鼠BP肠袢中AMPK和pAMPK mRNA表达水平,QRT-PCR法检测大鼠炎症相关因子白细胞介素1β(IL-1β)、白细胞介素6(IL-6)和肿瘤坏死因子α(TNF-α)、核因子κB(NF-κB)和白细胞介素10(IL-10)mRNA表达水平。结果:与术前比较,术后2周开始DJB组大鼠FBG水平明显降低(t=3.798,P0.05);与假手术组比较,DJB组大鼠FBG水平也明显降低(t=3.205,P0.05)。术后6周,与假手术组比较,DJB组大鼠术后HOMA-IR明显降低(t=4.441,P0.05),HOMA-ISI明显升高(t=-8.65,P0.05)。HE染色,与假手术组比较,DJB组大鼠BP肠袢肠管炎性细胞形态明显改善。QRT-PCR法检测,与假手术组比较,DJB组大鼠BP肠袢中促炎性因子IL-1β、IL-6、TNF-α和NF-κB表达水平明显降低(P0.05),而抗炎性因子IL-10表达水平明显升高(P0.05)。免疫组织化学检测,与假手术组比较,DJB组大鼠BP肠袢中AMPK及pAMPK表达水平明显升高(P0.05)。结论:DJB可明显改善T2DM大鼠血糖稳态及胰岛素抵抗,其机制可能与DJB改善T2DM大鼠BP肠袢的炎症状态、调控炎症因子的表达和活化AMPK分子有关。
[Abstract]:Objective: to investigate the effects of duodenal jejunal bypass (DJB) on the improvement of blood glucose homeostasis, insulin resistance and inflammation in obese type 2 diabetes mellitus (T2DM) rats. Methods: twenty ZDF rats were randomly divided into DJB group and sham operation group. 10 rats in each group, 8 rats survived in each group, the serum insulin levels were measured by Roche blood glucose analyzer and Elisa respectively at 1 week before operation and 2 weeks and 6 weeks after operation. HOMA-ISI (insulin sensitivity index) and HOMA-IRI (insulin resistance index) were calculated in rats. Six weeks after operation, the rats were killed to observe the inflammatory cell morphology of BP intestinal loop by HE staining. The expression level of AMPK and pAMPK mRNA in rat intestinal loops of BP was observed by immunohistochemical method. The expression of interleukin-1 尾 -1 尾 -1 尾 (IL-6), TNF- 伪, NF- 魏 BNF- 魏 B and IL-10IL-10 mRNA were detected by QRT-PCR in the intestinal loops of rats. The expressions of IL-1 尾, IL-6, TNF- 伪, NF- 魏 BNF- 魏 B) and IL-10IL-10 mRNA were detected by QRT-PCR. Results: compared with preoperative, From 2 weeks after operation, the level of FBG in DJB group was significantly lower than that in sham operation group, and the FBG level in DJB group was also significantly lower than that in sham operation group. At 6 weeks after operation, the postoperative HOMA-IR in DJB group was significantly lower than that in sham operation group, and HOMA-ISI was significantly higher in DJB group than in sham operation group. Compared with sham operation group, the morphology of inflammatory cells in BP intestinal loop of rats in DJB group was significantly improved by QRT-PCR. Compared with sham operation group, the expression levels of pro-inflammatory factor IL-1 尾, IL-6, TNF- 伪 and NF- 魏 B in the intestinal loop of rats in DJB group were significantly decreased, while the expression of anti-inflammatory factor IL-10 was significantly increased in DJB group. Compared with the sham operation group, the expression of AMPK and pAMPK in the intestinal loop of rats in DJB group was significantly higher than that in the control group (P 0.05). Conclusion the blood glucose homeostasis and insulin resistance of T2DM rats can be significantly improved by WJB. The mechanism may be similar to that of DJB in improving the inflammatory state of BP intestinal loop in T2DM rats. Regulation of the expression of inflammatory factors is related to activation of AMPK molecules.
【作者单位】：潍坊医学院药理学教研室应用药理学山东省高校重点实验室;山东省潍坊市人民医院小儿外科;
【基金】：国家自然科学基金课题(31671208) 山东省科技厅自然基金资助课题(ZR2015HL128)
【分类号】：R587.1;R656.6

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