雌激素预防性给药对去卵巢大鼠骨和脏器的影响

发布时间：2018-03-07 22:12

本文选题：骨质疏松　切入点：去卵巢　出处：《中国骨质疏松杂志》2017年12期 　论文类型：期刊论文

【摘要】：目的采用双侧卵巢切除术建立绝经后骨质疏松(postmenopausal osteoporosis,PMOP)大鼠模型,探讨雌激素预防性给药对绝经后大鼠骨和脏器的影响。方法将SD大鼠分为假手术(sham-operated,Sham)组、去卵巢(ovariectomized,OVX)组、雌二醇组(β-estradiol-treated OVX,OVX/E2)组。术后第10 d开始皮下注射给药并称量大鼠体重,术后61 d处死大鼠,取脏器和骨,称量脏器重量,计算脏器指数。制备组织切片,进行HE染色。结果组织形态学观察表明,OVX组大鼠的股骨和胫骨均出现骨小梁断裂、间距变大、结构紊乱等骨质疏松症状,而OVX/E2组并未出现明显的发病症状。相较于Sham组,OVX组大鼠子宫内膜固有层中的子宫腺数目增多,腺腔增大,子宫黏膜上皮明显增厚,而OVX/E2组的大鼠子宫形态结构并未发生明显病变。大鼠体重和脏器指数分析表明,摘除卵巢不仅会引起大鼠术后早期的体重增加,还会导致大鼠肝、肺、肾和脾的脏器指数增加,而雌激素预防性给药能一定程度上缓解去卵巢手术引发的脏器指数的异常变化。结论适时进行一定剂量的雌激素给药能够较好地预防绝经后骨质疏松症的发生,为绝经后骨质疏松症的预防和治疗提供参考。
[Abstract]:Objective to establish a rat model of postmenopausal osteoporosis (PMOP) by bilateral ovariectomy, and to explore the effects of prophylactic estrogen administration on bone and viscera of postmenopausal rats. Methods SD rats were divided into sham-operated sham group and ovariectomized OVX group. In the 尾 -estradiol-treated OVX / E2 group, the rats were injected subcutaneously on the 10th day after operation, and the rats were killed 61 days after operation. The organs and bones were taken, the viscera weight was weighed, the organ index was calculated, and tissue sections were prepared. Results histomorphologic observation showed that the femur and tibia of OVX group had bone trabecular fracture, the distance between them became larger, and the structure was disordered, and other symptoms of osteoporosis were found in OVX group. Compared with the Sham group, the number of uterine glands in the lamina propria of endometrium, the lumen of glands and the thickening of uterine mucosal epithelium were increased in OVX/E2 group. Body weight and organ index analysis showed that the removal of ovary not only caused early weight gain, but also increased organ index of liver, lung, kidney and spleen of rats in OVX/E2 group. The prophylactic administration of estrogen can alleviate the abnormal changes of visceral index caused by ovariectomized surgery to some extent. Conclusion timely administration of estrogen with a certain dose of estrogen can prevent postmenopausal osteoporosis. To provide a reference for the prevention and treatment of postmenopausal osteoporosis.
【作者单位】：陕西理工大学维生素D生理与应用研究所;陕西理工大学生物科学与工程学院;
【基金】：陕西省教育厅专项科研计划项目(15JK1127) 陕西省重点科技创新团队(2012KTC-29) 陕西理工大学科研基金项目(SLGQD13(2)-23)
【分类号】：R580

【相似文献】