Th22细胞及其功能分子IL-22在狼疮性肾炎中的作用研究

发布时间：2018-03-10 23:47

本文选题：Th细胞　切入点：IL-　出处：《中国免疫学杂志》2017年05期 　论文类型：期刊论文

【摘要】：目的:观察狼疮性肾炎(LN)患者外周血中的Th22细胞及其功能分子白细胞介素(IL)-22的表达,并探讨其意义。方法:选择系统性红斑狼疮患者(无肾脏受累)38例,狼疮性肾炎患者32例,健康志愿者10例,分别作为SLE组、LN组和健康对照组(HC组)。应用流式细胞仪检测其外周血中Th22细胞,酶联免疫吸附实验检测患者血清中IL-22的水平。比较三组Th22细胞和血清中IL-22水平差异,分析LN组Th22细胞和IL-22与SLE疾病活动指数评分(SLEDAI)的相关性。结果:SLE组和LN组患者外周血中Th22细胞较健康对照组明显升高(P0.01),血清中IL-22水平也较健康对照组明显升高(P0.01);Th22和IL-22在LN组较SLE组要稍微高表达,但差异均无统计学意义(P0.05);LN组患者Th22细胞比例和IL-22水平与对应患者的疾病活动性评分SLEDAI呈正相关。结论:Th22细胞和其功能分子IL-22参与SLE和LN的发病和进展。
[Abstract]:Objective: to observe the expression of Th22 cell and its functional molecule interleukin-22 in peripheral blood of patients with lupus nephritis and to explore its significance. Methods: 38 patients with systemic lupus erythematosus and 32 patients with lupus nephritis were selected. Ten healthy volunteers were used as SLE group LN group and healthy control group as HC group. Flow cytometry was used to detect Th22 cells in peripheral blood. Enzyme linked immunosorbent assay (Elisa) was used to detect the level of IL-22 in serum of patients. The correlation between Th22 cells and IL-22 in LN group and SLE activity index score (SLEDAI) was analyzed. Results the level of Th22 cells in peripheral blood of patients with LN and LN was significantly higher than that of healthy controls, and the level of IL-22 in serum was significantly higher than that in healthy controls. And IL-22 expression was slightly higher in LN group than in SLE group. However, there was no significant difference in the proportion of Th22 cells and the level of IL-22 in patients with LN, and there was a positive correlation between the percentage of Th22 cells and the level of IL-22 in the patients with LN. Conclusion the proportion of Th22 cells and their functional molecules IL-22 are involved in the pathogenesis and progression of SLE and LN.
【作者单位】：武汉科技大学医院内科;武汉科技大学附属天佑医院肾病科;枣阳市第一人民医院检验科;
【分类号】：R593.242

【相似文献】