ω-3多不饱和脂肪酸对去势大鼠骨微结构的改善

发布时间：2018-03-16 00:32

本文选题：ω-多不饱和脂肪酸　切入点：骨质疏松　出处：《中国骨质疏松杂志》2017年01期 　论文类型：期刊论文

【摘要】：目的观察ω-3多不饱和脂肪酸(PUFA)对去势大鼠骨微结构的影响。方法将30只雌性大鼠随机分为假手术组、模型组和实验组。假手术组仅切除卵巢周围脂肪组织,其余两组切除双侧卵巢。模型组和假手术组给予1 m L/(100 g·d)的生理盐水灌胃,实验组给予ω-3 PUFA 150 mg/(kg·d)。干预12 w后,处死所有大鼠,检测血钙、磷、碱性磷酸酶(ALP)和雌激素水平。分离右侧整段股骨应用micro-CT检测骨密度和骨微结构。结果雌二醇在3组之间存在显著差异,表现为模型组水平最低(8.1±2.8)mmol/L,而假手术组水平最高(25.0±8.9)mmol/L(P0.05)。实验组松质骨骨密度[(0.180±0.022)g/cm3vs(0.135±0.017)g/cm3]、BV/TV[(36.422±10.893)%vs(29.631±9.705)%]、Tb.Th[(0.138±0.044)mm vs(0.117±0.039)mm]和Tb.N[(2.252±0.078)/mm vs(1.589±0.083)/mm]均显著高于模型组,而Tb.Sp[(0.404±0.020)mm vs(0.599±0.032)mm]显著低于模型组(P0.05);实验组皮质骨骨密度[(0.718±0.015)g/cm3vs(0.633±0.025)g/cm3]、BV/TV[(48.559±7.152)%vs(33.878±8.764)%]和Cr.Th[(0.483±0.037)mm vs(0.428±0.034)mm]均显著高于模型组,而孔隙率[(32.162±7.891)%vs(38.859±9.847)%]显著低于模型组(P0.05)。结论ω-3PUFA对去卵巢大鼠的骨微结构有一定的改善作用,这对于防治骨质疏松具有一定的意义。
[Abstract]:Objective to observe the effect of 蠅 -3 polyunsaturated fatty acid (PUFAA) on bone microstructure in ovariectomized rats. Methods Thirty female rats were randomly divided into sham-operation group, model group and experimental group. The other two groups were ovariectomized. The model group and sham operation group were given 1 mL / L / 100g 路d normal saline intragastrically, and the experimental group was given 蠅 -3 PUFA 150 mg/(kg 路d. After 12 weeks of intervention, all the rats were killed and their blood calcium and phosphorus were detected. Alkaline phosphatase (ALP) and estrogen levels. Bone mineral density (BMD) and bone microstructure were measured by micro-CT in the right femur. Results there were significant differences in estradiol between the three groups. The bone mineral density of cancellous bone in the experimental group [0.180 卤0.022 g / cm ~ (-3) vs 0.135 卤0.017 mg / cm ~ 3] was significantly higher than that in the model group [36.422 卤10.893VS ~ (29.631 卤9.705)] Tb.Th [0.138 卤0.044 mm vs(0.117 卤0.039 mm] and Tb.N [2.252 卤0.078 mm vs(1.589 卤0.083 mm 卤0.033 mm] were significantly higher than that in the model group [0.404 卤0.020 vs(0.599 卤0.032 mm]; the cortical bone density in the experimental group was significantly higher than that in the model group [30.018 卤0.718 mm vs(0.117 卤0.039 mm] and Tb.N [2.252 卤0.078 mm vs(1.589 卤0.083 mm] was significantly higher than that in the model group [0.404 卤0.020 vs(0.599 卤0.032 mm]; the cortical bone density in the experimental group was significantly higher than that in the model group [0.404 卤0.020 vs(0.599 卤0.032 mm]; the experimental group [0.138 卤0.044 mm vs(0.117 卤0.039 mm] and Tb.N [2.252 卤0.078 mm vs(1.589 卤0.083 mm] were significantly higher than the model group [0.404 卤0.020 vs(0.599 卤0.032 mm]. The porosity (38.859 卤9.847%) was significantly lower than that in the model group (P 0.05). Conclusion 蠅 -3PUFA can improve the bone microstructure of ovariectomized rats, which has a certain significance for the prevention and treatment of osteoporosis.
【作者单位】：昆明医科大学第二附属医院肾脏内科;温州市中心医院外科;
【分类号】：R580

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