膳食钙对饮水型氟暴露致子代肝脏损伤的干预作用

发布时间：2018-03-16 09:40

本文选题：氟中毒　切入点：钙　出处：《毒理学杂志》2017年05期 　论文类型：期刊论文

【摘要】：目的探讨膳食钙对氟暴露致子代肝脏损伤的分子机制。方法取初断乳SD大鼠雌鼠100只,雄鼠50只。雌鼠随机分成对照、染氟、低钙、低钙染氟、高钙染氟5组。3个月后合笼,取14和28日龄仔鼠进行相关实验。结果与对照组相比,其他各组仔鼠肝细胞的凋亡数量明显增多,28日龄仔鼠低钙染氟组Bcl-2蛋白表达降低(P0.05),染氟组和低钙染氟组14日龄雄性、28日龄雌雄仔鼠肝组织Caspase12蛋白表达水平升高(P0.01);与染氟组相比,低钙染氟组的细胞凋亡数量明显增多,高钙染氟组的细胞凋亡数量有所下降,各日龄仔鼠低钙染氟组Bcl-2蛋白表达降低(P0.05),28日龄雄性仔鼠高钙染氟组Caspase12蛋白表达降低(P0.05),低钙组降低(P0.01)。结论氟中毒致子代肝损伤的机制可能是氟暴露导致子代肝组织上调内质网途径凋亡通路中Caspase12表达,下调Bcl-2的表达,低钙与染氟表现出协同作用,高钙则能缓解氟中毒症状。
[Abstract]:Objective to investigate the molecular mechanism of liver injury induced by dietary calcium exposure to fluoride. Methods 100 female SD rats and 50 male rats were randomly divided into control group, low calcium group, low calcium fluoride group and high calcium fluoride group. The 14 and 28 day old rats were selected to carry out the related experiments. The results were compared with those of the control group. The number of hepatocytes apoptosis in other groups increased significantly. The expression of Bcl-2 protein decreased in the low calcium fluoride group of 28 days old rats, while the expression level of Caspase12 protein in the liver tissue of the 14 day old male male rats exposed to fluorine and the low calcium fluoride group increased significantly (P 0.01), compared with the fluoride group, the expression of Caspase12 protein in the liver tissue of the rats exposed to fluoride was significantly higher than that of the control group. The number of apoptosis increased significantly in low calcium fluoride group, but decreased in high calcium fluoride group. The expression of Bcl-2 protein decreased in the low calcium fluoride group and the low calcium group in 28 days old male rats. Conclusion the mechanism of liver injury induced by fluorosis may be fluorine exposure induced by fluorosis in offspring liver tissue. Upregulate the expression of Caspase12 in the endoplasmic reticulum pathway. The expression of Bcl-2 was down-regulated. Low calcium and fluoride showed synergistic effect, while high calcium could relieve the symptoms of fluorosis.
【作者单位】：浙江师范大学化学与生命科学学院;浙江师范大学体育与健康科学学院;浙江师范大学行知学院;
【基金】：国家自然科学基金(81273015、81573101)
【分类号】：R595

【相似文献】