蛋白激酶C活性与糖尿病足截肢患者胫前动脉钙化积分的相关性

发布时间：2018-03-21 07:24

本文选题：糖尿病足　切入点：动脉粥样硬化　出处：《中国动脉硬化杂志》2017年04期 　论文类型：期刊论文

【摘要】：目的探讨蛋白激酶C(PKC)活性与糖尿病足截肢患者胫前动脉钙化之间的相关性。方法选取2012年10月至2016年1月在江苏大学附属医院骨科行糖尿病足大截肢住院治疗的患者共60例,根据CT对胫前动脉钙化程度的检测将患者分为轻度钙化组(n=20)、中度钙化组(n=20)及重度钙化组(n=20)。通过患者临床基线资料的采集以及胫前动脉组织连续石蜡切片Von Kossa染色观察钙化演进,通过PepTag~汶Assay法检测组织细胞膜PKC活性,通过Western blot检测对PKCα亚型进行半定量分析。结果糖尿病足截肢患者胫前动脉Von Kossa染色与相应的动脉CT扫描结果一致:胫前动脉斑块内的钙盐沉积呈点灶状分布,而且随着胫前动脉内斑块的不断扩大乃至管腔闭塞,钙颗粒的密度也有不同程度的增加,但始终呈点灶状分布的微钙化。定量检测及Pearson相关性分析显示PKC活性与糖尿病足胫前动脉钙化积分正相关(r=0.931,P0.001),进一步分析显示可能是PKCα亚型发挥了促进钙化演进的作用。结论 PKCα亚型的活化可能与糖尿病动脉斑块内钙化的演进存在正相关,其水平可能对针对性干预血管钙化有着一定的价值。
[Abstract]:Objective to investigate the correlation between protein kinase C kinase (PKC) activity and calcification of anterior tibial artery in diabetic foot amputation patients. Methods 60 patients with diabetic foot amputation were treated with diabetic foot amputation from October 2012 to January 2016 in Department of Orthopaedics, affiliated Hospital of Jiangsu University. The patients were divided into mild calcified group, moderate calcified group and severe calcified group according to CT examination of the calcification degree of anterior tibial artery. The clinical baseline data were collected and the Von Kossa staining of the tibial anterior artery tissue was used to observe the progress of calcification. The PKC activity of tissue membrane was measured by PepTag-Wen Assay assay. Results the results of Von Kossa staining of anterior tibial artery in diabetic foot amputation were consistent with those of CT scan. The calcium deposition in the plaque of anterior tibial artery was focal distribution. Moreover, with the enlargement of plaque in anterior tibial artery and the occlusion of lumen, the density of calcium granules increased in varying degrees. Quantitative analysis and Pearson correlation analysis showed that PKC activity was positively correlated with calcification integral of anterior tibial artery of diabetic foot. Further analysis showed that PKC 伪 subtype played a role in promoting calcification progression. Conclusion the activation of PKC 伪 subtype may be positively related to the progression of calcification in diabetic artery plaque. Its level may have certain value for intervention of vascular calcification.
【作者单位】：江苏大学附属医院病理科;江苏大学附属医院心内科;
【基金】：国家自然科学基金项目(81370408、81370409) 江苏省自然科学基金项目(BK20131246) 江苏省卫生厅项目(Q201308) 镇江市社会发展项目(SH2015038、SH2015023、SHW2015001) 卫生部核医学重点实验室江苏省分子核医学重点实验室开放课题(KF201504) 江苏大学临床专项项目(JDLCZX001)
【分类号】：R587.2

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