茶多酚对百草枯中毒大鼠肾脏氧化应激及炎症反应的影响

发布时间：2018-03-22 01:38

本文选题：茶多酚　切入点：百草枯　出处：《中国医科大学学报》2017年03期 　论文类型：期刊论文

【摘要】：目的探讨茶多酚对百草枯中毒大鼠肾脏氧化应激及炎症反应的影响及可能机制。方法将大鼠随机分为对照组(A组)、百草枯染毒组(B组)、百草枯染毒+小剂量茶多酚组(C组)、百草枯染毒+大剂量茶多酚组(D组),每组10只。HE染色观察肾脏组织,生化检测仪检测肌酐水平,ELISA法检测血清中8-异前列腺素、白细胞介素6(IL-6)及肿瘤坏死因子α(TNF-α)水平,Western blotting检测3-硝基酪氨酸(3-NT)蛋白水平,比色法检测肾脏组织氧化应激水平,实时PCR检测肾脏组织TNF-αmRNA及IL-6 mRNA水平。结果 B、C、D组大鼠在百草枯染毒后均出现肾脏受损,但C、D组在茶多酚干预下肾脏损伤较B组有所改善,且大剂量茶多酚干预下的D组肾脏损伤改善更为明显。在茶多酚干预下的C、D组血清中肌酐、8-异前列腺素、IL-6及TNF-α水平以及肾脏组织的丙二醛、3-NT、TNF-αmRNA及IL-6 mRNA水平较B组均降低,大剂量茶多酚干预下的D组降低更为显著。结论茶多酚干预对百草枯中毒大鼠肾脏有保护作用,其作用机制可能与减轻肾脏中氧化应激及炎症反应有关。
[Abstract]:Objective to investigate the effects of tea polyphenols on oxidative stress and inflammatory reaction in the kidney of paraquat poisoned rats. Methods Rats were randomly divided into control group A, paraquat group B and paraquat exposed to low-dose tea. Methods the rats were randomly divided into three groups: control group (group A), paraquat group (group B) and paraquat group (group B). Phenol group (group C), paraquat group (group D) treated with high dose tea polyphenols (group D), each group (10 rats) were stained with HE to observe the renal tissue. Serum levels of 8-isoprostaglandin (IL-6), interleukin-6 (IL-6) and tumor necrosis factor- 伪 (TNF- 伪) in serum were detected by biochemical assays. The levels of 3-nitrotyrosine 3-NT-1 protein were detected by Western blotting, and oxidative stress levels in renal tissues were detected by colorimetric method. Real-time PCR was used to detect the levels of TNF- 伪 mRNA and IL-6 mRNA in renal tissue. Results the renal damage was found in all rats in group B after paraquat exposure, but the renal injury was improved in group C (C) with tea polyphenols as compared with that in group B (P < 0.05). The levels of serum creatinine 8-isoprostaglandin IL-6 and TNF- 伪, and the levels of malondialdehyde 3-NTN TNF- 伪 mRNA and IL-6 mRNA in renal tissue in group C were lower than those in group B. Conclusion Tea polyphenols can protect the kidney of paraquat poisoned rats, and its mechanism may be related to the reduction of oxidative stress and inflammation in the kidney.
【作者单位】：中国医科大学附属盛京医院急诊科;中国医科大学附属盛京医院内分泌科;
【基金】：卫生部国家临床重点专科建设项目(2012-649)
【分类号】：R595.4

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