吸烟加重自发性高血压大鼠骨质疏松的风险

发布时间：2018-03-23 15:02

本文选题：吸烟　切入点：高血压　出处：《遵义医学院》2017年硕士论文

【摘要】：目的:通过影像学及病理组织学方法探讨吸烟是否加重自发性高血压大鼠骨质疏松的风险及可能机制。方法:选取12只健康的11周龄雄性WKY大鼠和12只11周龄雄性SHR大鼠,将WKY大鼠和SHR大鼠暴露于新鲜空气或烟草污染的空气中,分为4组,每组6只。A组:对照组(WKY+新鲜空气);B组:吸烟组(WKY+烟草暴露);C组:高血压组(SHR+新鲜空气);D组:吸烟联合高血压组(SHR+烟草暴露)。利用Micro-CT扫描检测大鼠第四腰椎的骨密度(BMD)、骨皮质厚度(Ct.Th)、骨体积分数(BV/TV)、骨小梁厚度(Tb.Th)、骨小梁间隙(Tb.Sp)等相关参数;HE染色观察腰椎骨小梁结构、骨细胞、脂肪细胞等骨形态学指标的变化;免疫组化观察腰椎HIF-1α、VEGF的表达情况。Image-Pro Plus6.0(IPP6.0)图像分析软件对HIF-1α和VEGF的平均光密度、骨小梁面积(Tb.Ar)、脂肪细胞进行半定量分析。SPSS17.0软件对结果进行统计学分析,P0.05表示具有显著性差异。结果:1.大鼠腰椎Micro-CT检测得出:与A组相比,B、C、D组的BMD、BV/TV、Ct.Th、Tb.Th下降,Tb.Sp升高,且具有显著性差异(P0.05);D组分别与B、C组相比,其BMD、BV/TV、Ct.Th、Tb.Th下降,Tb.Sp升高,且具有显著性差异(P0.05);而B、C组两组间的BMD、BV/TV、Ct.Th、Tb.Th、Tb.Sp无显著性差异(P0.05)。2.大鼠腰椎HE染色观察骨组织形态:A组骨小梁结构整齐、均匀一致,骨小梁之间连接紧密,骨小梁间隙较小,无发生断裂现象,骨细胞排列整齐,大小均一,细胞核均染,核无固缩;B、C组骨小梁粗细不一,骨小梁拱形结构破坏、可见骨小梁断裂现象,骨小梁间隙变宽,镜下可见骨细胞排列紊乱、大小不一,细胞核固缩,可见少量脂肪细胞;D组骨小梁结构严重破坏,骨小梁稀疏,骨细胞排列紊乱,哈弗氏管内可见血栓形成,髓腔内被大量脂肪细胞填充,且脂肪细胞形态不一,相互融合。利用IPP6.0软件对Tb.Ar、脂肪细胞数进行分析结果显示:与A组相比,B、C、D组的Tb.Ar百分比下降、脂肪细胞数增多,且差异均具有显著性(P0.05);D组分别与B、C组相比,其Tb.Ar百分比下降、脂肪细胞数增多,且差异均具有显著性(P0.05)。3.大鼠腰椎免疫组化HIF-1α、VEGF表达情况:各组大鼠的HIF-1α在骨细胞及髓腔内不同程度地表达;VEGF在髓腔中不同程度地表达。IPP6.0软件分析结果显示:与A组相比,D组HIF-1α和VEGF的平均光密度降低,且差异具有显著性(P0.05);与C组相比,D组HIF-1α和VEGF的平均光密度降低,且差异具有显著性(P0.05);A与C两组间和B与C两组间的HIF-1α平均光密度及VEGF平均光密度均无显著性差异(P0.05)。结论:1.吸烟和高血压均可使大鼠的骨密度下降、骨组织微结构破坏。2.吸烟可加速高血压大鼠骨密度下降、骨组织微结构破坏,加重骨质疏松发生的风险。3.吸烟使高血压大鼠的HIF-1α、VEGF表达减低,使骨形成受抑制并使骨内局部血运受损,从而加重骨量丢失,增加骨质疏松发生的风险。
[Abstract]:Objective: to investigate the risk and possible mechanism of osteoporosis in spontaneously hypertensive rats by means of imaging and histopathology. Methods: twelve healthy 11-week-old male WKY rats and 12 11-week-old male SHR rats were selected. WKY rats and SHR rats were exposed to fresh air or tobacco-polluted air and were divided into 4 groups. Group A: control group WKY fresh air group B: smoking group WKY tobacco exposure group C group: hypertension group SHR fresh air group D group: smoking combined with hypertension group SHR tobacco exposure. The bone of the fourth lumbar vertebra of rats was detected by Micro-CT scan. Density of BMD, thickness of bone cortex, bone volume fraction (BV / TVV), trabecular thickness, trabecular thickness, and trabecular space were observed by HE staining. The changes of bone morphology, such as bone cells and adipocytes, the expression of HIF-1 伪 in lumbar vertebrae by immunohistochemistry. The average optical density of HIF-1 伪 and VEGF by image-Pro Plus6.0) image analysis software. The bone trabecular area and adipocytes were analyzed by semi-quantitative analysis. SPSS 17.0 software showed significant difference in the results (P0.05). Results: 1. Micro-CT detection of lumbar vertebrae in rats showed that BMDV / TV-TVTT. Tb.Th in group A decreased Tb.Sp, and Tb.Sp increased in group B (P < 0.05). There were significant differences in BMDV BV / Tb. Tb. Th, Tb. Tb. Th and Tb.Sp, and there was no significant difference in BMDV BV / TVCt.ThTb.Sp between two groups (P < 0. 05), but there was no significant difference in BMDBV / TVCt.ThTb.Sp between two groups (P < 0. 05. 2). The structure of bone trabeculae in group A was observed by HE staining of lumbar vertebrae, and the structure of bone trabeculae in group A was observed by HE staining, and there was no significant difference between group B and group C (P < 0. 05), but there was no significant difference between group B and group B (P < 0. 05). The bone trabeculae were closely connected, the trabecular space was small, there was no fracture, the bone cells were arranged neatly, the size was uniform, the nuclei were stained, the trabeculae in group C were different in thickness, and the arch structure of bone trabeculae was destroyed. We can see the phenomenon of bone trabecular fracture, bone trabecular gap becoming wider, bone cell arrangement disorder, different size, nucleus pyknosis, small amount of adipocyte D group bone trabecular structure serious destruction, bone trabecula sparse, bone cell arrangement disorder, see a small number of adipocytes D bone trabecular structure serious destruction, bone trabeculae sparse, bone cell arrangement disorder. Thrombosis was observed in the Hafer's tube, and the medullary cavity was filled with a large number of adipocytes. The number of Tb.Ar. adipocytes was analyzed by IPP6.0 software. The results showed that the percentage of Tb.Ar in group A was lower than that in group A. The number of adipocytes increased, and the percentage of Tb.Ar decreased and the number of adipocytes increased in group D, compared with group B C, respectively. The expression of HIF-1 伪 in lumbar vertebrae: the expression of HIF-1 伪 in bone cells and intramedullary lumen of each group was different degrees. The results of software analysis showed that: compared with group A, the expression of HIF-1 伪 in medullary cavity was different from that in group A. Compared with group D, the average optical density of HIF-1 伪 and VEGF decreased, The average optical density of HIF-1 伪 and VEGF in group D was lower than that in group C, and the difference was significant (P 0.05), and the average optical density of HIF-1 伪 and VEGF in group D was lower than that in group C. There was no significant difference in the mean optical density of HIF-1 伪 and VEGF between group A and group C and between group B and C. Conclusion: 1. Smoking and hypertension can decrease the bone mineral density of rats. 2. Smoking can accelerate the decrease of bone mineral density and increase the risk of osteoporosis in hypertensive rats. Smoking can decrease the expression of HIF-1 伪 in hypertensive rats. The formation of bone is inhibited and local blood flow is damaged, which increases the loss of bone mass and increases the risk of osteoporosis.
【学位授予单位】：遵义医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R580;R544.1

【相似文献】