高原红细胞增多症血浆差异蛋白组学的初步研究

发布时间：2018-03-23 17:20

  本文选题：高原红细胞增多症　切入点：串联质谱标签　出处：《青海大学》2017年硕士论文

【摘要】：目的:应用串联质谱标签(TMT)结合质谱技术比较高原红细胞增多症(HAPC)患者和健康对照者血浆中的差异表达蛋白。方法:收集HAPC患者血浆4例,与之相匹配的健康对照组血浆5例,组内等量混合后利用多重免疫亲和层析柱去除14种高丰度蛋白,TMT试剂标记后样本进行液相色谱分离,采用HPLC-MS/MS质谱鉴定及相对定量;HAPC患者和健康对照各20例血浆,ELISA方法验证筛选的差异蛋白。结果:共鉴定和定量了1094种蛋白,其中差异表达的蛋白有249种,其中HAPC组较健康对照组表达量上调≥1.5倍的蛋白质有162种,表达量下调≤0.67倍的蛋白质有87种,炎性相炎症相关差异表达蛋白17种;炎症蛋白CRP和SAA在HAPC组中较健康对照组表达量均上调(P0.05)。结论:筛选出17种与炎症相关的差异表达蛋白,为深入研究HAPC发生机制奠定了良好基础。
[Abstract]:Objective: to compare the differentially expressed proteins in plasma of patients with high altitude polycythemia (HAP) and healthy controls by tandem mass spectrometry (tandem mass spectrometry) and mass spectrometry. Methods: plasma samples from 4 patients with HAPC and 5 healthy controls were collected. The samples were separated by liquid chromatography after being mixed in the same amount within the group, 14 kinds of high abundance protein TMT reagent were removed by multiple immunoaffinity chromatography column, and the samples were labeled with TMT reagent. The differential proteins were identified by HPLC-MS/MS mass spectrometry and 20 patients and 20 healthy controls by Elisa respectively. Results: 1094 proteins were identified and quantified, of which 249 were differentially expressed. In HAPC group, there were 162 kinds of protein whose expression was more than 1.5-fold, 87 kinds of protein with down-regulation 鈮，

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