双膦酸盐局部注入新西兰兔椎体内的早期实验性研究

发布时间：2018-03-25 05:11

本文选题：唑来膦酸　切入点：骨质疏松　出处：《皖南医学院》2015年硕士论文

【摘要】：目的:研究新西兰兔骨质疏松椎体内局部注射唑来膦酸的早期效果。方法:24只6月龄雌性新西兰白兔双侧去卵巢去势造骨质疏松模型,低钙饮食3个月后,行双能X线骨密度仪扫描,检测骨密度,确认骨质疏松模型成功。将骨质疏松模型新西兰白兔随机分为治疗组(A组)和对照组(B组)各12只,每组再分为3小组。治疗组(A1、A2、A3组)于兔第4腰椎椎体注入唑来膦酸1mg,骨蜡封闭,对照组(B1、B2、B3组)手术暴露出第4腰椎,同样方法穿刺成功未注药,缝合。两组术后正常饲养,观察动物一般情况。术后4周、8周、12周,经耳缘静脉空气栓塞法处死新西兰兔取材(椎体及其附件完整取出,包括上下各一椎体)。标本大体形态观察;双能X线定量分析骨密度;免疫组化染色;Micro CT扫描分析骨组织形态改变。所有数据采用统计学软件SPSS17.0进行分析。结果:卵巢切除术后12周,骨密度扫描结果显示骨质疏松动物模型建立成功。椎体注入药物术后未出现脱毛、腹泻及体重明显变化。唑来膦酸局部注入新西兰兔椎体后4周,X线片显示随着治疗时间的延长,片中高亮度区越明显,分布面积越广,而对照组则相反;组化染色显示,实验组较对照组的骨皮质厚度、骨小梁密度、骨小梁数目较对照组增加,骨小梁分布较对照组均匀,差异有统计学意义(P0.05)。Micro-CT分析显示治疗组骨小梁厚度(Tb.Th)、骨小梁数目(Tb.N)、相对骨体积(BV/TV)明显高于对照组,差异有统计学意义(P0.05),表面体积比和骨小梁间隙低于对照组;注射后8周效果更明显,持续到12周依然有效,差异仍有统计学意义(P0.05)。在不同时期,治疗组(A组)的骨小梁厚度、骨小梁数目、表观骨密度、相对骨体积明显高于对照组(B组)。结论:骨质疏松椎体单次注入唑来膦酸可快速促进骨皮质形成及骨小梁的改变,改善骨骼微结构,提高骨强度,作为临床局部治疗骨质疏松及骨质疏松性骨折的新方法,为临床局部治疗骨质疏松提供实验依据。
[Abstract]:Objective: to study the early effect of intravertebral injection of zoledronic acid into the osteoporotic vertebrae of New Zealand rabbits. Methods: Osteoporosis model was established in 24 6-month-old female New Zealand white rabbits after bilateral ovariectomy. Dual energy X-ray absorptiometry was performed to detect bone mineral density (BMD), and to confirm the success of osteoporosis model. New Zealand white rabbits were randomly divided into two groups: treatment group A (n = 12) and control group B (n = 12). Each group was subdivided into 3 groups. Treatment group A _ 1 A _ 1 A _ 2A _ 2 A _ 3) Zoledronic acid was injected into the fourth lumbar vertebra of rabbits, bone wax was blocked, and the control group (B _ (1) B _ (2) B _ (2) B _ (3)) exposed the fourth lumbar vertebrae. After 4 weeks, 8 weeks and 12 weeks after operation, the rabbits were killed by transauricular vein air embolization (the vertebrae and its appendages were removed completely, including the upper and lower vertebrae and the upper and lower vertebrae. The gross morphology of the specimens was observed. Dual energy X-ray quantitative analysis of bone mineral density; immunohistochemical staining micro CT scanning analysis of bone tissue morphology. All data were analyzed by statistical software SPSS17.0. Results: 12 weeks after ovariectomy, Bone mineral density scan showed that the animal model of osteoporosis was established successfully. 4 weeks after local injection of zoledronic acid into the vertebra of New Zealand rabbits, X-ray showed that with the prolongation of the treatment time, the higher the luminance area in the slice was, the wider the distribution area was, while the contrary was observed in the control group, and the histochemical staining showed that, Compared with the control group, the thickness of bone cortex, the density of trabecular bone, the number of trabeculae were increased, and the distribution of trabecular bone in the experimental group was more uniform than that in the control group. The difference was statistically significant (P 0.05) .Micro-CT analysis showed that the trabecular thickness and the number of trabeculae were significantly higher in the treatment group than in the control group, and the relative bone volume and relative bone volume were significantly higher than those in the control group. The surface volume ratio and trabecular space were lower in the treatment group than in the control group. The effect was more obvious at 8 weeks after injection, and continued to be effective at 12 weeks, and the difference was still statistically significant (P 0.05). At different stages, the trabecular thickness, the number of trabeculae, and the apparent bone mineral density of the treatment group (A group) were significantly higher than those of the control group. Conclusion: single injection of zoledronic acid into the osteoporotic vertebral body can promote the formation of bone cortex and the change of bone trabecula, improve the bone microstructure and increase the bone strength. As a new method for local treatment of osteoporosis and osteoporotic fracture, it provides experimental basis for clinical local treatment of osteoporosis.
【学位授予单位】：皖南医学院
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R580

【相似文献】