Roux-en-Y胃旁路术对肥胖合并2型糖尿病大鼠肝脏糖脂代谢的影响

发布时间：2018-03-29 08:21

本文选题：胆汁酸　切入点：法尼醇X受体　出处：《上海交通大学学报(医学版)》2017年09期

【摘要】：目的·通过构建肥胖合并2型糖尿病(T2DM)Roux-en-Y胃旁路手术(RYGB)大鼠模型,探讨RYGB对大鼠肝脏糖脂代谢的影响及其机制。方法·将大鼠随机分为正常对照组、高脂饮食+DM组、假手术组、手术组,每组10只。监测大鼠体质量、进食量、腹腔糖耐量试验(IPGTT)以及胰岛素耐量试验(ITT)结果的变化,并检测法尼醇X受体(FXR)、成纤维细胞生长因子21(FGF21)、葡萄糖-6-磷酸酶(G6Pase)的m RNA和蛋白表达水平。结果·与高脂饮食+DM组和假手术组比较,手术组大鼠体质量和进食量明显下降,IPGTT、ITT曲线下面积明显减小;肝脏油红O染色可见手术组大鼠红色脂滴较高脂饮食+DM组和假手术组明显减少;手术组的FXR、FGF21 m RNA表达明显升高,G6Pase m RNA表达降低;手术组FXR、FGF21蛋白表达明显升高,G6Pase蛋白表达降低。结论·RYGB可以激活FXR-G6Pase通路,有效改善肥胖合并T2DM大鼠的糖脂代谢。
[Abstract]:Objective to investigate the effects of RYGB on hepatic glucose and lipid metabolism in rats with obesity and type 2 diabetes mellitus (T2DMX Roux-en-Y) by gastric bypass surgery. Methods Rats were randomly divided into normal control group, high fat diet DM group and sham operation group. The changes of body mass, food intake, intraperitoneal glucose tolerance test (IPGTT) and insulin tolerance test (ITT) were monitored in 10 rats in each group. The expression levels of m RNA and protein in FXRN, FGF21, G6 Pasein, FGF21 and G6 Pasein were determined, and the results were compared with those in DM group and sham operation group. The area under the ITT curve of IPGTT was significantly decreased in the operation group, and the red lipid droplets in the operation group were significantly lower than those in the DM group and the sham-operation group, and the liver oil red O staining showed that the red lipid droplets in the operation group were lower than those in the high fat diet DM group and sham-operation group. The expression of FXR- FGF21 m RNA was significantly increased in the operation group and decreased in the operation group, and the expression of FXR- FGF21 protein increased significantly in the operation group. Conclusion RYGB can activate the FXR-G6Pase pathway and effectively improve the glycolipid metabolism in the obese rats with T2DM.
【作者单位】：南方医科大学附属奉贤区中心医院普外科;上海市糖尿病研究所上海交通大学附属第六人民医院内分泌代谢科;
【基金】：上海市卫生与计划生育委员会科研课题(201440026) 上海市教育委员会高峰高原学科建设计划(20152527)~~
【分类号】：R587.1;R589.2;R656.6

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