2型糖尿病相关代谢标志物的筛选探究

发布时间：2018-03-30 01:03

本文选题：型糖尿病　切入点：尿液代谢指纹图谱　出处：《现代预防医学》2017年01期

【摘要】：目的研究空腹血糖受损的2型糖尿病前期人群和2型糖尿病新发患者尿液代谢指纹谱的变化模式,筛选出可能与2型糖尿病相关的潜在代谢标志物。方法利用气相色谱-质谱联用(Gas Chromatograph-Mass Spectrometer,GC-MS)技术对正常人群、空腹血糖受损的2型糖尿病前期人群和2型糖尿病新发患者尿液代谢指纹谱进行测定;运用多元统计方法对GC-MS数据进行分析,筛选出与2型糖尿病可能相关潜在代谢标志物。结果 2型糖尿病新发患者尿液代谢指纹谱的个体差异较正常人群和前期人群增大;正常人群、前期人群和新发患者尿液代谢指纹谱具有差异;初步筛选出三种可能与2型糖尿病相关的潜在代谢标志物D-氨基葡萄糖酸(Glucosoxime)、棕榈酸(Hexadecanoic acid)和β-假尿苷(β-pseudouridine)。结论本研究初步筛选出了可能与2型糖尿病相关的潜在尿液代谢标志物,证实了GC-MS技术联合偏最小二乘判别分析(Partial Least Squares-Discriminant Analysis,PLS-DA)多元统计分析应用于2型糖尿病早期发现和疾病状态监控的可行性。
[Abstract]:Objective to study the patterns of urine metabolic fingerprints in type 2 diabetes patients with impaired fasting blood glucose, and to screen out potential metabolic markers related to type 2 diabetes mellitus.Methods the urine metabolic fingerprints were determined by gas chromatography-mass spectrometry (GC-MS) in normal subjects, pre-type 2 diabetes patients with impaired fasting blood glucose and newly diagnosed type 2 diabetes mellitus (T2DM).GC-MS data were analyzed by multivariate statistical method and potential metabolic markers associated with type 2 diabetes were screened.Results the individual difference of urine metabolic fingerprint spectrum of new type 2 diabetes mellitus patients was higher than that of normal group and early stage group, and there was difference between normal group, early stage group and new patient group, and there was significant difference in urine metabolic fingerprint spectrum between normal group, early stage group and newly developed group.Three potential metabolic markers, D-glucosamine, Hexadecanoic acid) and 尾 -pseudouridine (尾 -pseudouridine) were preliminarily screened.Conclusion this study has preliminarily screened out potential urinary metabolic markers associated with type 2 diabetes mellitus.The feasibility of GC-MS combined with partial Least Squares-Discriminant Analysis (PLS-DAA) for early detection of type 2 diabetes mellitus and monitoring of disease status was confirmed.
【作者单位】：四川大学华西公共卫生学院(华西第四医院);四川省疾病预防控制中心;
【分类号】：R587.1

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