沙格列汀对高糖环境下成骨细胞增殖、分化和凋亡的影响

发布时间：2018-04-01 17:17

  本文选题：糖尿病性骨质疏松症　切入点：沙格列汀　出处：《山东医药》2017年28期

【摘要】：目的观察沙格列汀对高糖环境下成骨细胞增殖、分化和凋亡的影响,为糖尿病性骨质疏松患者选择合理的降糖药物提供理论依据。方法取成骨细胞MC3T3-E1置于a-MEM培养基中常规培养、传代。取传3代的对数生长期细胞,随机分为正常对照组、高糖组、高糖联合沙格列汀组,即刻更换a-MEM培养基,正常对照组更换为葡萄糖浓度5.5 mmol/L的培养液,高糖组更换为葡萄糖浓度30.0 mmol/L的培养液,高糖联合沙格列汀组更换为葡萄糖浓度30.0 mmol/L+沙格列汀1μmol/L的培养液,继续培养48 h。收集各组细胞,分别采用CCK-8法和流式细胞术检测细胞增殖能力和细胞凋亡率,ELISA法检测碱性磷酸酶(ALP)活性,qRT-PCR法检测成骨细胞分化相关基因Ⅰ型胶原(COL-Ⅰ)、成骨特异性转录因子2(Runx2)、骨钙蛋白(OCN)、骨桥蛋白(OPN)mRNA表达。结果与正常对照组比较,高糖组、高糖联合沙格列汀组细胞增殖能力、ALP活性及成骨细胞分化相关基因COL-Ⅰ、Runx2、OCN、OPN mRNA相对表达量均明显降低,细胞凋亡率均明显升高(P均0.05);与高糖组比较,高糖联合沙格列汀组细胞增殖能力、ALP活性和成骨细胞分化相关基因COL-Ⅰ、Runx2、OCN、OPN mRNA相对表达量均明显升高,细胞凋亡率均明显降低(P均0.05)。结论沙格列汀能在一定程度上逆转高糖环境对小鼠成骨细胞增殖、分化和凋亡的影响,为糖尿病性骨质疏松患者的降糖治疗提供了理论依据。
[Abstract]:Objective to observe the effects of salgletine on the proliferation, differentiation and apoptosis of osteoblasts in high glucose environment, and to provide a theoretical basis for the selection of appropriate hypoglycemic drugs in diabetic osteoporosis patients. Three passages of logarithmic growth cells were randomly divided into normal control group, high glucose group, high glucose combined with salglutin group, and a-MEM medium was changed immediately. The normal control group was replaced with 5. 5 mmol/L glucose culture medium. The high glucose group was replaced with glucose concentration of 30.0 mmol/L, and the high glucose combined with salglutin group with glucose concentration of 30 mmol/L and 1 渭 mol/L. The cells of each group were collected and cultured for 48 hours. CCK-8 assay and flow cytometry were used to detect cell proliferation ability and apoptosis rate. Elisa was used to detect alkaline phosphatase (ALP) activity and qRT-PCR was used to detect osteoblast differentiation related gene type I collagen, osteoblast-specific transcription factor 2G Runx 2, bone. The expression of osteopontin, osteopontin, and osteopontin mRNA were compared with those in normal control group. In high glucose group and high glucose combined with salglutin group, the activity of ALP and the relative expression of osteoblast differentiation related gene COL- 鈪，

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