三磷酸腺苷敏感性钾离子通道在外源性硫化氢抑制高糖诱导的成骨细胞损伤中的作用

发布时间：2018-04-01 17:04

本文选题：硫化氢　切入点：高糖　出处：《华西口腔医学杂志》2017年05期

【摘要】：目的研究三磷酸腺苷敏感性钾离子通道(KATP)在硫化氢(H2S)抑制高糖(HG)诱导的成骨细胞损伤中的作用。方法原代培养大鼠下颌骨成骨细胞并鉴定,将成骨细胞给予HG、H2S、KATP通道开放剂吡拉地尔(Pia)、阻断剂格列本脲(Gli)处理后,用Western blot检测KATP通道蛋白的表达水平,同时采用CCK8、实时逆转录聚合酶链反应(RT-PCR)、茜素红S染色分析方法检测其对成骨细胞增殖、分化及矿化的影响。结果细胞KATP通道蛋白的表达水平在HG的影响下明显降低,H2S预处理明显抑制HG对KATP通道蛋白的下调作用和对成骨细胞增殖的影响,并防止HG引起的成骨细胞分化和矿化的减少;相反,KATP通道阻断剂能明显阻断H2S对成骨细胞的上述保护作用。结论 H2S通过KATP通道抑制了HG诱导的成骨细胞损伤。
[Abstract]:Objective to study the role of adenosine triphosphate sensitive potassium ion channel (KATP) in inhibiting osteoblast injury induced by HG). Methods Primary cultured rat mandibular osteoblasts were identified. After the osteoblasts were treated with piradil and glibenclamide, the Western blot was used to detect the expression of KATP channel protein. At the same time, CCK8, real-time reverse transcriptase polymerase chain reaction (RT-PCR) and alizarin red S staining were used to detect the proliferation of osteoblasts. Results under the influence of HG, the expression level of KATP channel protein significantly decreased. H2S pretreatment significantly inhibited the down-regulation of KATP channel protein and the proliferation of osteoblasts by HG. On the contrary, KATP channel blocker could significantly block the above protective effect of H2S on osteoblasts. Conclusion H2S can inhibit the osteoblast injury induced by HG through KATP channel.
【作者单位】：潍坊医学院口腔医学院潍坊医学院附属医院口腔科;潍坊医学院临床医学院;解放军89医院骨科;
【基金】：国家自然科学基金(31570941,31270993) 教育部新世纪人才计划(NCET-10-0922) 山东省医药卫生科技计划(2016-WS0684) 山东省高等学校科技计划(J14LK12) 潍坊市科技发展计划(2016YX066) 潍坊医学院教师公派国内访学项目~~
【分类号】：R587.2;R782

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