黄芪乌梅配方颗粒对高脂饲料联合地塞米松诱导大鼠胰岛素抵抗的影响

发布时间：2018-04-08 10:52

本文选题：黄芪　切入点：乌梅　出处：《甘肃中医药大学(原名：甘肃中医学院)》2015年硕士论文

【摘要】：目的:观察黄芪(Astragalus)乌梅(Dark Plum)配方颗粒对高脂饲料(High fat diet,Hyperlipid diet,HFD)联合地塞米松(Dexamethasone,DEX)诱导的胰岛素抵抗大鼠血糖水平、血脂代谢水平的影响,以及肝脏胰岛素受体m RNA和骨骼肌GLUT4 m RNA表达情况,探讨黄芪乌梅配方颗粒治疗胰岛素抵抗的作用及其机制。方法:选取雄性Wistar大鼠40只,4-6周龄,体重240±20g,随机数表法选取35只后,高脂饲料喂养的同时隔日皮下注射地塞米松(1mg·kg-1)。注射结束后测定空腹血糖,规定空腹血糖升高且糖耐量异常的大鼠为造模成功大鼠。将造模后大鼠随机分为三组:模型组、黄芪乌梅颗粒给药组、阳性药物对照组(参芪降糖颗粒),另设正常对照组(n=5)。血糖试纸法检测血糖,IPGTT法检测葡萄糖耐量,试剂盒检测血脂(TC、TG)、血清游离脂肪酸(FFA)、血清胰岛素(FINS)水平,HE染色法观察大鼠肝脏病理改变,RT-PCR检测胰岛素受体m RNA和GLUT4m RNA表达。结果:1、模型组空腹血糖高于正常饮食组(p0.05);葡萄糖耐量试验模型组负荷后血糖曲线下面积高于正常对照组(p0.05);黄芪乌梅配方颗粒治疗组大鼠空腹血糖出现明显下降(p0.05),胰岛素抵抗指数(Homa-IR)显著下降(p0.05)。2、模型组TC和FFA水平均明显高于正常对照组(p0.05),黄芪乌梅配方颗粒治疗后大鼠TC和FFA出现显著下降(p0.05)。3、肝脏切片染色后显示模型组的肝组织呈肝索排列紊乱及水肿样病理改变;黄芪乌梅配方颗粒给药组肝细胞病理改变较模型组病理改变较轻微。4、模型组大鼠肝脏组织的胰岛素受体m RNA和骨骼肌组织的GLUT4 m RNA表达量低于正常组(p0.05),黄芪乌梅配方颗粒干预后大鼠胰岛素受体m RNA和GLUT4m RNA表达量有所上调。结论:黄芪乌梅配方颗粒能有效地降低胰岛素抵抗模型大鼠的血糖,纠正脂代谢紊乱,改善高脂血症,其机制可能与上调肝脏组织中胰岛素受体m RNA以及骨骼肌中GLUT4m RNA表达有关。
[Abstract]:Objective: to observe the effects of Astragalus Astragalus Dark Plumum (Astragalus) on blood glucose and lipid metabolism in rats with insulin resistance induced by high fat dietate (HFDs) and dexamethasone (DEX).The expression of insulin receptor m RNA in liver and GLUT4 m RNA in skeletal muscle were studied to explore the effect and mechanism of Huangqi Wumei recipe granule in the treatment of insulin resistance.Methods: 40 male Wistar rats aged 4 to 6 weeks, weight 240 卤20g, were randomly selected and fed with high fat diet. Dexamethasone 1mg kg-1g was injected subcutaneously every other day.Fasting blood glucose (FBG) was measured at the end of the injection, and the rats with elevated fasting blood glucose and abnormal glucose tolerance were designated as successful rats.The rats were randomly divided into three groups: model group, Huangqi Wumei granule group, positive drug control group (Shenqi Jiangtang granule, normal control group).Blood glucose test paper method was used to detect glucose tolerance, serum free fatty acid (FFAA) and serum insulin (FINS) levels were measured. The expression of insulin receptor m RNA and GLUT4m RNA in rat liver was detected by reverse transcription-polymerase chain reaction (RT-PCR).Results: 1, the fasting blood glucose in the model group was higher than that in the normal diet group, the area under the blood glucose curve in the glucose tolerance test group was higher than that in the normal control group after loading, and the fasting blood glucose of the rats in the Huangqi Wumei prescription treatment group was significantly decreased (P 0.05).The insulin resistance index (Homa-IRI) decreased significantly (p0.05). The levels of TC and FFA in the model group were significantly higher than those in the normal control group (P 0.05). The TC and FFA of the model group were significantly decreased after the treatment of Huangqi Wumei recipe granule. The liver sections stained showed the liver group of the model group.Disordered arrangement of hepatic cord and edematous pathological changes were observed.Compared with the model group, the pathological changes of hepatocytes in the Huangqi Wumei prescription granule group were lighter than those in the model group. The expression of insulin receptor m RNA and GLUT4 m RNA in the liver tissue and skeletal muscle tissue of the model group were lower than that in the normal group (P 0.05), and the expression of GLUT4 m RNA in the liver tissue of the model group was lower than that in the normal group.The expression of insulin receptor m RNA and GLUT4m RNA were up-regulated after the intervention of formula granule.Conclusion: Huangqi Wumei prescription granule can effectively reduce blood sugar, correct lipid metabolism disorder and improve hyperlipidemia in rats with insulin resistance.The mechanism may be related to the up-regulation of insulin receptor m RNA in liver and GLUT4m RNA expression in skeletal muscle.
【学位授予单位】：甘肃中医药大学(原名：甘肃中医学院)
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R587.1

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