胰岛素受体在猕猴腰段脊髓及相应背根神经节中的分布

发布时间：2018-04-09 15:50

  本文选题：胰岛素受体　切入点：脊髓背角　出处：《神经解剖学杂志》2017年05期

【摘要】：目的:观察胰岛素受体在猕猴脊髓腰段第4~6(L4~L6)节段及相应节段背根神经节(dorsal root ganglion,DRG)中的形态学分布特点。方法:取1只成年猕猴的L4~L6节段脊髓并留取相应节段的DRG置于固定液中。脊髓冷冻水平切片(30μm)平均分为4组,分别行Nissl染色、荧光免疫组织化学染色、免疫组织化学染色和对照组处理,再于光学显微镜下观察;DRG平均分为2组,分别行免疫组织化学染色和对照组处理,再于光学显微镜下观察。结果:胰岛素受体分布于猕猴腰段脊髓L4~L6节段及相应节段的DRG中:(1)在腰段脊髓L4~L6节段,背角可见胰岛素受体阳性神经元胞体及终末,并大量分布于背角浅层(集中在第I、II层),腹角也可观察到散在胰岛素受体阳性神经元;(2)在L4~L6节段对应的DRG中,胰岛素受体阳性产物主要集中分布于中型(20μm直径≤40μm)、小型(直径≤20μm)神经元。结论:本研究为进一步研究灵长类动物脊髓和DRG中胰岛素受体的功能提供形态学依据。
[Abstract]:Aim: to observe the morphological distribution of insulin receptor in dorsal root ganglion (DRGs) of dorsal root ganglia (DRGs) and its corresponding segments in the lumbar spinal cord of rhesus monkey.Methods: the spinal cord of the L4~L6 segment of an adult rhesus monkey was taken and the DRG of the corresponding segment was taken and placed in the fixed solution.The chilled spinal cord was divided into 4 groups: Nissl staining, fluorescent immunohistochemical staining, immunohistochemical staining and control group.Immunohistochemical staining and control group were performed respectively, and then observed under optical microscope.Results: the insulin receptor was distributed in the L4~L6 segment of the rhesus monkey's lumbar spinal cord and in the DRG of the corresponding segment. In the L4~L6 segment of the lumbar spinal cord, the cell bodies and terminals of insulin receptor positive neurons were found in the dorsal horn of the spinal cord.And distributed in the superficial layer of the dorsal horn (concentrated in the second layer of Ignus II and scattered in the ventral horn of the insulin-receptor-positive neuron Angi2) in the DRG corresponding to the L4~L6 segment, and a large number of them were distributed in the superficial layer of the dorsal horn.The positive products of insulin receptor were mainly distributed in the middle sized neurons (20 渭 m in diameter 鈮，

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