磁标记内皮祖细胞移植对糖尿病大鼠肾功能改善的作用:MRI成像验证

发布时间：2018-04-12 07:03

本文选题：干细胞 + 糖尿病　；参考：《中国组织工程研究》2017年05期

【摘要】：背景:近几年的诸多研究发现,内皮祖细胞在损伤性疾病等方面有重要作用。目的:探讨磁标记内皮祖细胞移植对糖尿病大鼠肾功能的改善作用及MRI成像。方法:将60只Wistar大鼠随机分为3组,正常对照组不作任何处理;模型组、实验组腹腔注射40 mg/kg链脲佐菌素,建立1型糖尿病模型,造模后4周,实验组尾静脉注射磁标记内皮祖细胞悬液0.15 m L(细胞浓度1×109 L-1)。细胞移植8周后,检测3组大鼠空腹血糖、血清胰岛素、血肌酐和尿素氮及24 h尿蛋白水平;采用MRI对移植细胞进行活体示踪,并与肾脏组织切片进行对照;测量大鼠体质量及肾质量变化,计算肾质量指数。结果与结论:(1)与正常对照组比较,模型组空腹血糖、血肌酐和尿素氮、24 h尿蛋白及肾质量指数升高(P0.05),胰岛素水平降低(P0.05);(2)与模型组比较,实验组空腹血糖、血肌酐、尿素氮、24 h尿蛋白及肾质量指数降低(P0.05),胰岛素水平升高(P0.05);(3)实验组可见稍长T1短T2信号,病灶边缘部位明显,梯度回波FLASH序列较T2加权RARE序列明显,其余组均未见明显低信号改变;实验组可见磁标记的阳性细胞且与组织切片结果相吻合,模型组和正常对照组未见磁标记的阳性细胞;(4)结果表明,磁标记内皮祖细胞移植可在一定程度上改善糖尿病大鼠的肾功能紊乱。
[Abstract]:Background: in recent years, many studies have found that endothelial progenitor cells play an important role in injurious diseases.Objective: to investigate the effect of magnetically labeled endothelial progenitor cell transplantation on renal function and MRI imaging in diabetic rats.Methods: sixty Wistar rats were randomly divided into 3 groups without any treatment in the normal control group, and in the experimental group, 40 mg/kg streptozotocin was injected intraperitoneally to establish the model of type 1 diabetes.The experimental group was injected with magnetic labeled endothelial progenitor cell suspension of 0.15 mL (cell concentration 1 脳 10 9 L -1).After 8 weeks of cell transplantation, fasting blood glucose, serum insulin, serum creatinine, urea nitrogen and 24 h urine protein levels were measured in three groups, and MRI was used to trace the transplanted cells in vivo and compared with renal tissue sections.The body mass and renal mass of rats were measured and the renal mass index was calculated.Results compared with the normal control group, the fasting blood glucose, serum creatinine and urea nitrogen nitrogen 24 h urinary protein and renal mass index in the model group were increased (P 0.05), the insulin level was decreased (P 0.05) and compared with the model group, the fasting blood glucose and serum creatinine in the model group were significantly higher than those in the control group.Urinary protein and renal mass index (RMI) were decreased in 24 h in urea nitrogen group (P 0.05) and insulin level was increased (P 0.05). In the experimental group, a little longer T 1, short T 2 signal was observed, the edge of the lesion was obvious, and the gradient echo FLASH sequence was more obvious than that of T 2 weighted RARE sequence.There were no significant changes in low signal intensity in the other groups, and the positive cells were observed in the experimental group, which were consistent with the results of tissue sections, but not in the model group and the normal control group (P < 0.05).Magnetic labeled endothelial progenitor cell transplantation can improve renal dysfunction in diabetic rats to some extent.
【作者单位】：河北大学附属医院放射科;河北大学附属医院CT室;河北大学附属医院核医学科;
【基金】：国家自然科学基金面上项目(51473044)~~
【分类号】：R587.2;R692.9

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