TLR2基因敲除下调高脂饮食诱导的肥胖小鼠脂肪组织炎症因子表达和细胞凋亡

发布时间：2018-04-13 05:13

本文选题：肥胖 + 脂肪组织　；参考：《免疫学杂志》2017年02期

【摘要】：目的研究Toll样受体2(TLR2)基因敲除对高脂饮食诱导的肥胖小鼠脂肪组织炎症因子表达和细胞凋亡的影响。方法雄性C57BL6小鼠和TLR2基因敲除小鼠分为:正常对照组、肥胖组、TLR2基因敲除组和TLR2基因敲除肥胖组,并给予普通饮食或高脂饮食喂养。16周后检测各组小鼠空腹血糖、甘油三酯、总胆固醇、低密度脂蛋白、高密度脂蛋白和游离脂肪酸水平。分光光度计法检测小鼠脂肪组织Caspase3活性,Western blot检测Bcl2、Bax、MyD88和p65NFκB蛋白相对表达量。荧光实时定量PCR检测小鼠脂肪组织IL-6和TNF-αmRNA相对表达量。结果与正常对照组相比,肥胖组小鼠空腹血糖、甘油三酯、总胆固醇、低密度脂蛋白、高密度脂蛋白水平降低和游离脂肪酸水平升高;脂肪组织Caspase3活性、Bax蛋白、MyD88蛋白、p65NFκB蛋白、IL-6 mRNA和TNF-αmRNA相对表达量升高,Bcl2蛋白相对表达量减少。与肥胖组相比,基因敲除肥胖组小鼠空腹血糖、甘油三酯、总胆固醇、低密度脂蛋白、高密度脂蛋白水平降低和游离脂肪酸水平降低;脂肪组织Bcl2蛋白相对表达量增加,Caspase3活性、Bax蛋白、MyD88蛋白、p65NFκB蛋蛋白、IL-6 mRNA和TNF-αmRNA相对表达量减少。结论 TLR2基因敲除下调了高脂饮食诱导的肥胖小鼠脂肪组织炎症因子的表达和细胞凋亡。
[Abstract]:Objective to study the effects of Toll like receptor 2 (TLR2) knockout on the expression of inflammatory factors and apoptosis in adipose tissue of obese mice induced by high fat diet.Methods male C57BL6 mice and TLR2 knockout mice were divided into three groups: normal control group, obese group and obese group. Fasting blood glucose and triglyceride were measured after feeding with normal diet or high-fat diet for .16 weeks.Total cholesterol, low density lipoprotein, high density lipoprotein and free fatty acid levels.The activity of Caspase3 in adipose tissue of mice was detected by spectrophotometer and the relative expression of Bcl2Bax-MyD88 and p65NF 魏 B protein was detected by Western blot.The relative expression of IL-6 and TNF- 伪 mRNA in adipose tissue of mice was detected by fluorescence real time quantitative PCR.Results compared with the control group, the levels of fasting blood glucose, triglyceride, total cholesterol, low density lipoprotein, high density lipoprotein and free fatty acid increased in obese group.The relative expression of IL-6 mRNA and TNF- 伪 mRNA in adipose tissue was increased and the relative expression of Bcl2 protein was decreased.Compared with the obese group, the fasting blood glucose, triglyceride, total cholesterol, low density lipoprotein, high density lipoprotein and free fatty acid were decreased in the gene knockout group.The relative expression of Bcl2 protein in adipose tissue increased the activity of Caspase3 and decreased the relative expression of IL-6 mRNA and TNF- 伪 mRNA in p65NF 魏 B egg protein.Conclusion TLR2 knockout can down-regulate the expression of inflammatory factors and apoptosis in adipose tissue of obese mice induced by high fat diet.
【作者单位】：锦州医科大学附属第一医院内分泌科;锦州医科大学研究生学院;锦州医科大学附属第一医院普外科;
【基金】：辽宁省科技厅提供2013年博士启动基金(20131067) 2016年辽宁省自然科学基金(201602308)的资金支持
【分类号】：R589.2

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