EGCG调控骨骼肌组织TRB3表达改善胰岛素抵抗

发布时间：2018-04-13 22:13

  本文选题：胰岛素抵抗 + EGCG　； 参考：《西南师范大学学报(自然科学版)》2017年11期

【摘要】：目的:研究EGCG(Epigallocatechin Gallate)是否抑制骨骼肌组织的TRB3(Tribbles Homologue 3)表达,激活PI3K/AKT信号通路,促进骨骼肌对葡萄糖的摄取和利用.方法:80只SD(Sprague Dawley)大鼠随机分为正常对照组(20只)、模型对照组(20只)、EGCG低剂量治疗组(20只)和EGCG高剂量治疗组(20只).模型对照组、EGCG低剂量治疗组和EGCG高剂量治疗组给予高糖高脂饮食6个月后,EGCG低剂量治疗组和EGCG高剂量治疗组分别给予EGCG治疗,治疗4周和8周分别处死各组大鼠各半,检测血清中葡萄糖、胰岛素含量并计算胰岛素抵抗指数;检测骨骼肌组织TRB3和AKT的表达及AKT的磷酸化程度.结果:模型组中葡萄糖、胰岛素和胰岛素抵抗指数(p0.05),EGCG治疗4周和8周后,各指标均降低(p0.05);模型组中AKT的mRNA和蛋白质在各组中无差异,但P-AKT(473)在模型组表达下调,治疗后表达上调,8周治疗较4周明显(p0.05).模型组中TRB3的mRNA和蛋白质表达增加(p0.05),治疗后TRB3表达的下调,8周治疗较4周明显(p0.05);结论:EGCG可降低血糖,其机制可能与抑制TRB3的表达,增加AKT的磷酸化程度,激活PI3K/AKT信号通路,促进骨骼肌细胞对葡萄糖的摄取和利用,抑制胰岛素抵抗.
[Abstract]:Aim: to investigate whether EGCG(Epigallocatechin Gallate inhibits the expression of TRB3(Tribbles Homologue 3 in skeletal muscle, activates PI3K/AKT signaling pathway, and promotes glucose uptake and utilization in skeletal muscle.Methods 80 SD(Sprague Dawley rats were randomly divided into normal control group (n = 20), model control group (n = 20) and EGCG high dose group (n = 20).After 6 months of high glucose and high fat diet, the low dose group and the high dose group of EGCG were treated with EGCG respectively. The rats of each group were killed for 4 weeks and 8 weeks, respectively.The serum glucose, insulin content and insulin resistance index were measured. The expression of TRB3 and AKT and the phosphorylation of AKT in skeletal muscle were detected.Results: after 4 and 8 weeks of treatment, the levels of glucose, insulin and insulin resistance index were all decreased in model group, and the mRNA and protein of AKT in model group were not different in each group, but the expression of P-AKTn473) was down-regulated in model group.After treatment, the expression of upregulation was significantly increased in 8 weeks than in 4 weeks of treatment (P 0.05).In the model group, the expression of mRNA and protein in TRB3 increased p0.05, and the down-regulation of TRB3 expression in the model group was significantly higher than that in the 4th week after treatment. Conclusion the expression of mRNA and protein in the model group can be reduced by inhibiting the expression of TRB3, increasing the phosphorylation of AKT and activating the PI3K/AKT signaling pathway.Promote the uptake and utilization of glucose in skeletal muscle cells and inhibit insulin resistance.
【作者单位】： 云南省第三人民医院内分泌科;昆明医科大学生物化学与分子生物学系;昆明医科大学第一附属医院糖尿病科;昆明医科大学第一附属医院移植科;昆明学院医学院生物化学教研室;
【基金】：国家自然科学基金项目(81360128) 云南省应用基础研究基金项目(2013FD052,2015FB046) 云南省教育厅研究基金项目(2012C006,2013C081)
【分类号】：R587.1
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本文编号：1746423