百色市壮族血尿酸水平与代谢综合征各组分及脂肪因子ZAG的相关性研究

发布时间：2018-04-15 11:05

本文选题：高尿酸血症 + 锌α2糖蛋白　；参考：《右江民族医学院》2017年硕士论文

【摘要】：目的:本研究以广西百色市壮族人群作为研究对象,初步探讨壮族人群的尿酸水平与MS各组分(高血脂、高血压、高血糖、肥胖)及脂肪因子ZAG的相关性。通过构建高尿酸血症小鼠模型,进一步论证SUA与ZAG的相关性。为预防和控制慢性代谢性疾病提供科学依据,为HUA的防治提出新的方向。方法:1百色市壮族高尿酸血症患者的生化指标与代谢综合征各组分的相关性分析(1)抽取百色市所辖的右江区、田东县、田阳县、德保县、田林县、靖西县等地的市县区医院、右江民族医学院直属附属医院及附属西南医院体检者中,三代及三代以上均为壮族的人群进行调查;(2)对研究对象一般健康指标及生化指标进行检测;(3)根据所测的一般健康指标及生化指标,将受试者合并的代谢性疾病分为:肥胖、高血压、高血脂、高血糖。2百色市壮族尿酸水平与新脂肪因子ZAG的相关性分析(1)从第一部分2284例受试者中,随机抽取高尿酸血症患者150例,健康对照组受试者150例。经检查均无心、肝、肺、肾、脑等脏器疾病,无消化道和血液系统疾病史。年龄范围20-70岁。2组对象性别、年龄等基线资料比较差异无统计学意义(P0.05);(2)实时荧光定量PCR检测受试者全血ZAG mRNA表达水平;(3)ELISA法检测受试者血清ZAG蛋白浓度。3基于高尿酸血症小鼠模型探讨血尿酸与ZAG的相关性(1)尿酸酶抑制法构建高尿酸小鼠模型;(2)检测小鼠血清生化指标及ZAG蛋白浓度。结果:(1)二分类Logistics回归分析:代谢综合征危险组分(超重/肥胖、高血压、高血糖、高血脂)是壮族高尿酸血症的危险因素(P0.05或P0.001),是否合并以上各危险组分诊断高尿酸血症的ROC曲线下面积与0.5相比,差异有统计学意义(P0.001);(2)HUA组血清中ZAG的表达水平高于对照组(P0.001),且ZAG mRNA的相对表达水平也较高(P0.001);SUA与ZAG呈正相关(P0.01);ZAG诊断HUA的ROC曲线下面积为0.770,与0.5相比差异有统计学意义(P0.001);(3)小鼠SUA与BUN,CREA,ZAG,FPG,TC,TG,LDL-C呈正相关(P0.05或P0.001),与HDL-C呈负相关(P0.001);SUA与ZAG的一元线性回归方程为:Y(SUA)=-239.758+2.533X(ZAG)。结论:(1)壮族HUA受试者合并MS各组分的顺序依次为:高血脂,高血压,高血糖,肥胖;各组分也是壮族HUA的危险因素,是否合并各危险组分对壮族高尿酸血症的诊断效果欠佳,灵敏度较低;(2)壮族人群尿酸水平与ZAG呈正相关,血清ZAG浓度对高尿酸血症的诊断准确性一般;(3)尿酸酶抑制法可以成功诱导高尿酸小鼠模型;基于动物模型进一步验证了尿酸水平与ZAG呈正相关。
[Abstract]:Objective: to investigate the correlation between uric acid level and MS components (hyperlipidemia, hypertension, hyperglycemia, obesity) and fat factor ZAG in Zhuang population in Baise, Guangxi.The relationship between SUA and ZAG was further demonstrated by constructing hyperuricemia mouse model.To provide a scientific basis for the prevention and control of chronic metabolic diseases, and to provide a new direction for the prevention and treatment of HUA.Methods the correlation analysis of biochemical indexes and metabolic syndrome components in Zhuang patients with hyperuricemia in 1: 1) the hospitals in Youjiang District, Tiandong County, Tianyang County, Debao County, Tianlin County, Jingxi County, etc., which are under the jurisdiction of Baise City, were selected from the city and county hospitals of Baise City, such as Youjiang District, Tiandong County, Tianyang County, Debao County, Tianlin County, Jingxi County, etc.Among those under the affiliated Hospital of Youjiang Medical College for nationalities and the affiliated Southwest Hospital,A survey of the population of Zhuang nationality of the third generation and more than three generations was carried out. (2) the general health index and biochemical index of the subjects were tested. (3) according to the general health index and biochemical index, the metabolic diseases were divided into obesity, obesity, obesity, obesity, obesity, obesity, obesity, obesity, obesity, obesity, obesity, obesity, obesity, obesity, obesity, obesity, obesity and obesity.Analysis of correlation between the level of uric Acid and New Fat Factor ZAG in Zhuang nationality in the first part of the study, 150 patients with hyperuricemia and 150 healthy controls were randomly selected from the first part of the study.There was no history of diseases of digestive tract and blood system, such as heart, liver, lung, kidney, brain and so on.Age range 20-70 years. 2 groups of sex,There was no significant difference between age and baseline data (P 0.05) real-time quantitative PCR was used to detect the expression of ZAG mRNA in whole blood of subjects. The serum ZAG protein concentration was detected by Elisa. 3 based on the model of hyperuricemia mice, the relationship between serum uric acid and serum levels of serum ZAG protein was studied.Correlation of ZAG 1) the hyperuricemia mouse model was established by uric acid enzyme inhibition method. The serum biochemical indexes and the concentration of ZAG protein in mice were detected.Results Logistics regression analysis showed that the risk components of metabolic syndrome (overweight / obesity, hypertension, hyperglycemia),Hyperlipidemia) is a risk factor of hyperuricemia in Zhuang nationality (P 0.05 or P 0.001).There was a significant difference in the expression of ZAG in serum of P0.001HUA group compared with the control group, and the relative expression level of ZAG mRNA was also higher than that of the control group. There was a positive correlation between P0.001SUA and ZAG. The area under the ROC curve of the diagnosis of HUA by ZAG was 0.770, which was significantly higher than that of the control group.SUA was positively correlated with TGG LDL-C (P0.05 or P0.001C), and negatively correlated with HDL-C (P0.001SUA and ZAG). The linear regression equation between SUA and ZAG was: yam-SUA-239.758 2.533XZAGG.Conclusion (1) the sequence of MS components in Zhuang HUA subjects was as follows: hyperlipidemia, hypertension, hyperglycemia, obesity, components were also the risk factors of HUA in Zhuang nationality, the diagnostic effect of hyperuricemia in Zhuang was not good.There was a positive correlation between uric acid level and ZAG in Zhuang nationality population. The diagnostic accuracy of serum ZAG concentration for hyperuricemia was 3%) the mice model of hyperuricemia could be induced successfully by the method of inhibition of uric acid enzyme.The positive correlation between uric acid level and ZAG was further verified based on animal model.
【学位授予单位】：右江民族医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R589

【参考文献】