糖尿病足患者血清热激蛋白70的水平变化及其相关因素分析

发布时间：2018-04-15 12:20

  本文选题：糖尿病足 + 热激蛋白70　； 参考：《昆明医科大学》2017年硕士论文

【摘要】：[目的]通过观察未合并足部溃疡的糖尿病患者、合并有足部溃疡的糖尿病患者以及无糖尿病的正常对照组人群血清中热激蛋白70(HSP70)水平的变化,以及热激蛋白70(HSP70)与一些临床基本资料、生化指标的相关性,从而来初步探讨热激蛋白70(HSP70)在糖尿病及糖尿病足溃疡(DFU)发生、发展过程中的作用,为糖尿病足溃疡(DFU)早期防治提供一定的理论依据。[方法]选取2015年9月至2016年9月昆明总医院收治的2型糖尿病(T2DM)患者,将未合并足部溃疡的糖尿病患者30例(DM组)、合并有足部溃疡的糖尿病患者30例(DFU组)以及无糖尿病的正常对照组人群30例(NC组)分别纳入研究。测定所有入选对象空腹静脉血甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、糖化血红蛋白(HbA1c%)、空腹血糖(FPG)、餐后2小时血糖(2hPG)以及血清中热激蛋白70(HSP70)的水平,同时通过10g尼龙单丝试验、下肢血管超声检查对DM组和DFU组的周围神经病变及下肢血管病变情况进行评估,记录相关数据后最后进行对比分析。所有资料采用SPSS17.0软件,两组间计量资料比较采用t检验,计数资料比较采用x2检验,相关性分析采用Pearson、Spearman及多重线性回归相关分析法,P0.05为有统计学意义。[结果]①DM组的空腹静脉血中HbA1c、FPG、2hPG、HSP70水平明显高于NC组,存在显著差异(P0.01)。②DFU组的空腹静脉血中LDL-C、HbAlc%、FPG、2hPG、HSP70水平以及10g尼龙单丝检测、下肢血管超声检查结果的异常率均明显高于DM组,差异有统计学意义(P0.05);③DM组和DFU组患者的体重指数(BMI)、血LDL-C水平、10g尼龙单丝检测及下肢血管超声检查结果异常率与血清HSP70水平之间存在显著相关性(P0.05)。[结论]①血清热激蛋白70可能参与了糖尿病及糖尿病足部溃疡发生、发展的病理生理过程。②合理调节低密度脂蛋白胆固醇水平、控制体重,以及加强对糖尿病患者神经病变、血管病变的筛查可能一定程度上能预防和延缓糖尿病足部溃疡病变的发生发展。
[Abstract]:[objective] to observe the changes of serum heat shock protein 70 (HSP70) levels in diabetic patients without foot ulcers, diabetic patients with foot ulcers and normal controls without diabetes mellitus.The relationship between heat shock protein 70 (HSP70) and some basic clinical data and biochemical indexes was also discussed to explore the role of heat shock protein 70 (HSP70) in the pathogenesis and development of diabetes mellitus and diabetic foot ulcer.To provide a theoretical basis for early prevention and treatment of diabetic foot ulcer.[methods] Type 2 diabetes mellitus (T2DM) patients treated in Kunming General Hospital from September 2015 to September 2016 were selected.30 diabetic patients without foot ulcers, 30 diabetic patients with foot ulcers, 30 diabetic patients with foot ulcers (DFU group) and 30 normal controls without diabetes mellitus (NC group) were included in the study.The levels of triglyceride triglyceride (TG), total cholesterol (TC), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), glycosylated hemoglobin (HbA1cT), fasting blood glucose (FPGG), 2 hours postprandial blood glucose (2 hPGs) and serum heat shock protein (70 HSP70) were measured in all subjects.At the same time, 10 g nylon monofilament test and lower extremity vascular ultrasound were used to evaluate peripheral neuropathy and lower extremity vascular lesion in DM group and DFU group.All the data were analyzed by SPSS17.0 software. T test was used to compare the measurement data between the two groups and x2 test was used to compare the counting data. The correlation analysis was conducted by Pearsonian Spearman and multiple linear regression correlation analysis (P0.05).[results] the level of HSP70 in fasting venous blood of 1DM group was significantly higher than that of NC group. There was a significant difference in the level of HSP70 and 10 g nylon monofilament in fasting venous blood of 1DM group. The abnormal rate of lower extremity vascular ultrasound was significantly higher than that of DM group.There was a significant difference in body mass index (BMI) between P0.05DM-group and DFU group. There was a significant correlation between the abnormal rate of 10 g nylon monofilament in serum LDL-C level and the serum HSP70 level.[conclusion] 1Serum heat shock protein 70 may be involved in the development of diabetes mellitus and diabetic foot ulcers.Strengthening the screening of diabetic neuropathy and vascular disease may prevent and delay the occurrence and development of diabetic foot ulcerative disease to some extent.
【学位授予单位】：昆明医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R587.2

【参考文献】

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1 李峥t，

本文编号：1754084