慢性锰中毒大鼠5-羟色胺、脑源性营养因子的表达变化

发布时间：2018-04-15 18:20

本文选题：慢性锰中毒 + 大鼠　；参考：《广西医科大学》2015年硕士论文

【摘要】：目的研究慢性锰中毒大鼠脑组织的5-羟色胺(5-HT)、脑源性营养因子(BDNF)的表达变化,了解慢性锰中毒大鼠抑郁行为及锰蓄积对脑组织5-HT、BDNF表达的影响。方法取80只SPF级SD雄性大鼠,分为2组：对照组和实验组,实验组又分为三个亚组：低锰组、中锰组、高锰组,分别腹腔注射Mncl2.4H2O 5 mg/kg、15 mg/kg、25 mg/kg,对照组予注射等量的生理盐水,获得慢性锰中毒大鼠模型。于第12周采用蔗糖水偏好试验、敝箱实验评估大鼠是否具有抑郁样症状,电感耦合等离子体-原子发射光谱法(ICP-AES法)测定纹状体锰含量。运用高效液相色谱法测定额叶、海马5-HT含量,Western blot法检测额叶、海马的BDNF表达,并采用实时荧光定量PCR技术检测BDNF、5-HT的限速酶色氨酸羟化酶2 (TPH2) mRNA的表达情况。结果1、蔗糖消耗试验及敝箱实验结果显示,慢性锰中毒大鼠出现抑郁样行为,高剂量组表现更加明显,差别有统计学意义(均P0.05)。2、高效液相色谱法结果表明给锰组大鼠额叶及海马BDNF表达随锰浓度增加而下降明显,其中高锰组表达最低,呈剂量依赖性,差别有统计学意义(均P0.05)。3. Western blot结果表明给锰组大鼠额叶及海马BDNF表达随锰浓度增加而下降明显,其中高锰组表达最低,呈剂量依赖性,差别有统计学意义(均P0.05)。4、real-time PCR结果示锰暴露后大鼠额叶皮层及海马的TPH2mRNA, BDNFmRNA表达水平均降低,且与锰中毒程度相关联,呈剂量依赖性,差别有统计学意义(均P0.05)。结论1、慢性锰中毒大鼠有抑郁样行为存在；2、锰蓄积致额叶及海马神经元内5-HT、BDNF表达下调,慢性锰中毒大鼠伴发抑郁可能与脑组织5-HT、BDNF表达下调有关。
[Abstract]:Objective to study the chronic manganese poisoning rat brain serotonin 5- (5-HT), brain-derived neurotrophic factor (BDNF) expression, understanding chronic manganese poisoning Depression Behavior of rats and manganese accumulation of 5-HT on brain tissue, BDNF expression. Methods 80 SPF male SD rats were divided into 2 groups. The control group and the experimental group, the experimental group was divided into three subgroups: low manganese group, manganese group, high manganese group, intraperitoneal injection of Mncl2.4H2O 5 mg/kg, 15 mg/kg, 25 mg/kg, control group with normal saline injected, for chronic manganese poisoning rats. In twelfth weeks by sugarcane sucrose preference test, our experimental evaluation of rats with box depression like symptoms, inductively coupled plasma atomic emission spectrometry (ICP-AES) determination of manganese content in striatum. The use of HPLC method for the determination of 5-HT content in frontal lobe, hippocampus, frontal Western detection method of blot expression in hippocampus of BDNF, and a real Fluorescence quantitative PCR detection of BDNF, the rate limiting enzyme of tryptophan hydroxylase 2 (5-HT TPH2) expression of mRNA. Results 1, sucrose consumption test box and our experimental results show that the depression like behavior in rats of chronic manganese poisoning, the high dose group was more obvious, the difference was statistically significant (P0.05).2, the results showed that the HPLC method for manganese in rats and the expression of BDNF in hippocampus and frontal lobe decreased significantly with the increase of the concentration of manganese, high manganese group was the lowest in a dose-dependent manner, the difference was statistically significant (P0.05).3. Western blot analysis showed that Mn treated rats decreased the expression of BDNF in frontal lobe and hippocampus obviously when Mn concentration increased, the high manganese group was the lowest in a dose-dependent manner, the difference was statistically significant (P0.05).4, real-time PCR TPH2mRNA showed frontal cortex and hippocampus of rats exposed to manganese, the expression level of BDNFmRNA decreased, and Associated manganese poisoning, in a dose-dependent manner, the difference was statistically significant (P0.05). Conclusion: 1. Chronic manganese intoxication in rats with depression like behavior; 2, manganese accumulation induced by frontal and hippocampal neurons in 5-HT, BDNF expression, chronic manganese intoxication in rats with depression and brain tissue 5-HT. The down-regulation of BDNF.

【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R595.2

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