高压电烧伤对SD大鼠血栓调节蛋白、蛋白C、蛋白S及D二聚体的早期影响

发布时间：2018-04-15 21:22

本文选题：高压电烧伤 + 血栓调节蛋白　；参考：《实用医学杂志》2016年01期

【摘要】：目的:探讨高压电烧伤对SD大鼠外周血血栓调节蛋白(thronlbonlodulin,TM)、蛋白C(protein C,PC)、蛋白S(protein S,PS)及D二聚体(D-Dimer,D-D)的影响。方法:将120只SD大鼠按随机数字表法分为假高压电烧伤组(简称假伤组)、高压电烧伤组(简称电伤组),每组60只。2组致伤前15 min按随机数字表法各取10只大鼠,心脏采血分离血浆。电伤组余下50只大鼠用调压器和实验变压器造成高压电烧伤,假伤组余下50只大鼠按相同装置不通电模拟致伤。伤后5 min和1、2、4、8 h,每组SD大鼠按随机数字表法各取10只大鼠,心脏采血分离血浆。测定各时相点血清中TM、PC、PS及血浆中D-D的变化。对数据行析因设计方差分析、LSD检验。结果:电伤组SD大鼠的TM浓度在伤后5 min至8 h明显高于假伤组(均P0.05):除伤前15 min(P0.05)外,电伤组大鼠其余各时相点PC、PS浓度均低于假伤组(均P0.05):D-D浓度在伤后8 h达到最高值[(173.05±4.08)ng/mL]。结论:高压电烧伤早期,SD大鼠TM、D-D的浓度升高,PC、PS的浓度降低,血管内皮细胞的完整性被破坏,凝血功能受到严重影响。
[Abstract]:Objective: to investigate the effects of high voltage electric burn on the levels of thrombomodulin (TMP), C(protein, S(protein and D-Dimer D in peripheral blood of SD rats.Methods: 120 Sprague-Dawley rats were randomly divided into sham high voltage burn group (sham injury group) and high voltage electric burn group (electric injury group). Ten rats in each group were randomly selected 15 min before injury.The blood was taken from the heart to separate the plasma.The remaining 50 rats in the electric injury group were burned with voltage regulator and experimental transformer, while the remaining 50 rats in the sham injury group were injured without electrical simulation according to the same device.Ten SD rats in each group were randomly selected at 5 min and 4 h after injury. The blood samples were collected from the heart to separate the plasma.The changes of serum TMN PCPS and D D in plasma were measured at different time points.The data were analyzed by factorial analysis of variance (ANOVA) and LSD test.Results: the TM concentration of SD rats in electrical injury group was significantly higher than that in sham injury group from 5 min to 8 h after injury (all P 0.05: except 15 minutes before injury, P 0.05). The concentration of PS at other time points in electric injury group was lower than that in sham injury group (all P0.05):D-D concentration reached the highest value at 8 h after injury [173.05 卤4.08)ng/mL].Conclusion: the concentration of TMN D-D in SD rats was increased in the early stage of high voltage burn, the concentration of PCP decreased, the integrity of vascular endothelial cells was destroyed, and the coagulation function was seriously affected.
【作者单位】：西安交通大学医学院第二附属医院检验科;陕西省西北妇女儿童医院;河北医科大学第一医院;
【基金】：河北省医学科学研究重点课题计划(编号:20130563)
【分类号】：R647

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