基于iTRAQ技术在类风湿关节炎患者滑膜组织中差异表达蛋白的初步研究

发布时间：2018-04-17 18:51

本文选题：类风湿关节炎 + iTRAQ技术　；参考：《兰州大学》2017年硕士论文

【摘要】：目的本实验应用iTRAQ技术初步分析RA、OA及健康对照组患者滑膜组织中的差异表达蛋白,探讨这些差异表达蛋白及其所参与的代谢通路与RA发病的相关性,为研究RA的形成机理及临床干预提供理论依据。方法收集兰州大学第二医院2015年3月至2016年7月关节外科RA、OA及健康对照组患者(6例、14例、6例)手术后废弃的滑膜组织作为研究对象。应用iTRAQ技术对蛋白质进行定性、定量分析并对差异表达蛋白进行GO功能分析及KEGG pathway通路注释分析。结果1.差异表达蛋白定量分析显示:RA组与N组相较,发现395个差异表达蛋白,其中上调蛋白216个,下调蛋白179个;RA组与OA组相比较,存在421个差异表达蛋白,234个上调表达蛋白,187个下调表达蛋白;OA组与N组相比较,共发现326个差异表达蛋白,132个差异表达蛋白表达上调,94个差异表达蛋白下调。2.通过对差异表达蛋白的GO功能注释分析显示,RA组与N组相比较,发现生物学过程的功能分类有24种、分子功能作用有11种、分别位于10种细胞中。RA组与OA组相较存在25种不同的生物学过程、11种分子功能、分别位于10种细胞中。而OA组与N组比较,生物学过程有24种功能分类、分子功能作用有12种、位于11种细胞中。3.通过对差异表达蛋白的KEGG Pathway分析显示,RA组与N组、RA组与OA组、OA组与N组比较,分别存在35条、41条、20条信号通路。结论1.S100蛋白家族、FIB家族参与了RA的发生、发展,有望成为RA诊断的新的分子标志物。2.MMPs及CRP蛋白水平与RA患者病情活动程度相关。3.PREX1、APO C-I可能参与了RA疾病的发生、发展。4.PI3K-AKT信号通路在RA患者的治疗中具有潜在价值。
[Abstract]:Objective to analyze differentially expressed proteins in synovial tissues of patients with RAA OA and healthy controls by iTRAQ technique, and to explore the correlation between these proteins and the metabolic pathways involved in the pathogenesis of RA.To provide theoretical basis for studying the formation mechanism and clinical intervention of RA.Methods from March 2015 to July 2016, 14 patients with OA in arthrosurgery and 6 healthy controls were collected from the second Hospital of Lanzhou University. The abandoned synovial tissue was used as the study object.The protein was qualitatively and quantitatively analyzed by iTRAQ, and the differential expression protein was analyzed by go function analysis and KEGG pathway pathway annotation analysis.Result 1.Quantitative analysis of differentially expressed proteins showed that 395 differentially expressed proteins were found in group A and group N, including 216 up-regulated proteins and 179 down-regulated proteins in RA group compared with OA group.There were 421 differentially expressed proteins, 234 up-regulated proteins, and 187 down-regulated expression proteins compared with N group. 326 differentially expressed proteins were found in OA group, 132 differentially expressed proteins were up-regulated and 94 differentially expressed proteins were down-regulated.According to the go functional annotation analysis of differentially expressed proteins, it was found that there were 24 kinds of biological processes and 11 kinds of molecular functions in RA group compared with N group.There were 25 different biological processes and 11 molecular functions in RA group and OA group, respectively.Compared with N group, OA group had 24 functional classifications and 12 molecular functional functions, which were located in 11 kinds of cells.KEGG Pathway analysis of differentially expressed proteins showed that there were 35 or 41 signal pathways in RA group, N group, OA group, OA group and N group, respectively.Conclusion the 1.S100 family is involved in the pathogenesis and development of RA, which may be a new molecular marker for the diagnosis of RA. 2.MMPs and CRP protein levels may be related to the severity of disease activity in RA patients. 3. PREX1 APO C-I may be involved in the pathogenesis of RA.Development of PI3 K-AKT signaling pathway has potential value in the treatment of RA patients.
【学位授予单位】：兰州大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R593.22

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