氟对小鼠睾丸谷胱甘肽抗氧化系统的影响

发布时间：2018-04-17 23:34

本文选题：氟中毒 + 睾丸　；参考：《畜牧兽医学报》2017年05期

【摘要】：为探讨氟化钠对小鼠睾丸谷胱甘肽抗氧化系统的影响,将24只3周龄ICR雄性小鼠随机分为对照组和染氟组,对照组给予蒸馏水,染氟组分别供给含25、50、100mg·L~(-1) NaF的蒸馏水,自由饮用60d。染毒结束后检测小鼠睾丸总抗氧化能力(T-AOC)、活性氧(ROS)、丙二醛(MDA)、还原型谷胱甘肽(GSH)的含量及谷胱甘肽过氧化氢酶(GPx)、谷胱甘肽硫转移酶(GST)、谷胱甘肽还原酶(GR)的活性,检测GPx1、GPx4、GST-mu5和GR基因的转录。电镜观察睾丸组织细胞内线粒体、内质网和高尔基体的结构。结果显示,氟中毒可使睾丸T-AOC减弱,ROS、MDA含量升高,GSH含量显著降低,细胞内氧化水平升高,线粒体、内质网和高尔基体出现不同程度的损伤,GPx、GST、GR的活性降低,GPx1、GPx4、GST-mu5和GR基因转录下调。小鼠氟中毒后,睾丸抗氧化能力降低,处于明显的氧化应激状态,这与谷胱甘肽抗氧化系统酶活性降低及其相关基因转录下调有关。
[Abstract]:To investigate the effect of sodium fluoride on the antioxidant system of glutathione in testis of mice, 24 3-week-old male ICR mice were randomly divided into control group and fluorine-exposed group. The control group was given distilled water, and the fluorine-exposed group was given distilled water containing 2550100mg / L ~ (-1) NaF for 60 days.The contents of total antioxidant capacity (T-AOC), reactive oxygen species (Ros), malondialdehyde (MDAA), reduced glutathione (GSH) and the activities of glutathione catalase (GST), glutathione catalase (GST) and glutathione reductase (GRG) were measured after exposure.The transcription of GST-mu5 and gr genes in GPx1 and GPx4 was detected.The structure of mitochondria, endoplasmic reticulum and Golgi apparatus in testicular cells was observed by electron microscope.The results showed that fluorosis could significantly decrease the T-AOC content, increase the content of rosid MDA, increase the level of intracellular oxidation, and damage the mitochondria, endoplasmic reticulum and Golgi body. The activity of GST-GST-mu5 and gr gene were down-regulated by GST-mu5 and GST-mu5.After fluorosis, the antioxidant ability of testis decreased and the mice were in a state of obvious oxidative stress, which was related to the decrease of glutathione antioxidant system enzyme activity and the down-regulation of transcription of related genes.
【作者单位】：山西农业大学动物科技学院;
【基金】：国家自然科学基金(31201965) 山西农业大学青年拔尖创新人才支持计划项目(TYIT201408)
【分类号】：R599.1;S859.8

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