系统性红斑狼疮和干燥综合征患者血清白介素-33和骨桥蛋白水平的研究

发布时间：2018-04-19 03:15

本文选题：系统性红斑狼疮 + 干燥综合征　；参考：《昆明医科大学》2016年硕士论文

【摘要】：[目的]研究中国系统性红斑狼疮(SLE)和干燥综合征(SS)患者的血清中白介素-33(IL-33)和骨桥蛋白(OPN)的水平。[方法]研究对象共60例,所有的病例均来自昆明医科大学第二附属医院风湿免疫科门诊。包括男、女性,年龄16—74岁,平均年龄41岁,且分为4组。分别为(1)SLE组(31人)(2)SS组(8人)(3)SLE合并桥本甲状腺炎病组(12人)(4)健康对照组(9人)。用Quantikine ELISA human IL-33 immunoassay 和 Quantikine ELISA human osteopontin immunoassay试剂、ELISA方法测定四组11--33和OPN的血清浓度。[结果]1.我们发现四组之间血清IL-33和OPN水平没有显著差别。四组的血清IL-33水平F值和P值分别是0.144和0.933,P值0.05,差异没有统计学意义,因此四组间血清IL-33水平无明显差异。四组的血清OPN水平F值和P值分别是0.4602和0.006,P值0.05,差异有统计学意义,因此四组间血清OPN水平有明显差异。鉴于这种差异,我们进一步做了四组间的多重比较。结果发现,SLE合并桥本甲状腺炎病组的血清OPN的浓度水平比其他三组显著升高。2.IL-33和OPN与SLE疾病活动度间无明显差异(P0.05)(除了抗SSA-Ab阳性者),多数的临床和实验室变化与血清IL-33和OPN水平无关联。抗SSA-Ab阳性的SLE病人其血清IL-33水平更高,但其血清OPN水平与临床表现之间无相关性。IL-33和OPN与SS疾病活动度之间无明显差异(P0.05)(除了抗SSB-Ab阳性者)。多数的临床和实验室变化与血清IL-33和OPN水平无关联。抗SSB-Ab阳性的SS病人其血清OPN水平更高,但其血清IL-33水平与临床表现之间无相关性。[结论]血清IL-33水平在SLE、SS、SLE合并桥本甲状腺炎与正常对照无明显差异；而血清OPN水平各组间有明显差异,SLE合并桥本甲状腺炎的病人其血清OPN的浓度水平显著高于其他三组。
[Abstract]:[objective] to study the serum levels of interleukin-33 (IL-33) and osteopontin (OPN) in Chinese patients with systemic lupus erythematosus (SLE) and Sjogren's syndrome (SS).Methods A total of 60 cases were studied, all of whom came from the Department of Rheumatology and Immunology, the second affiliated Hospital of Kunming Medical University.Male, female, age 16-74 years old, average age 41 years, and divided into 4 groups.There were 31 patients with SLE and 8 patients with SLE complicated with Hashimoto's thyroiditis (n = 12) and 9 healthy controls (n = 9).Quantikine ELISA human IL-33 immunoassay and Quantikine ELISA human osteopontin immunoassay Elisa were used to determine the serum concentrations of 11-33 and OPN in four groups.[result] 1.We found no significant difference in serum IL-33 and OPN levels among the four groups.The F and P values of serum IL-33 in the four groups were 0.144 and 0.933, respectively. There was no significant difference between the four groups, so there was no significant difference in the serum IL-33 levels among the four groups.The levels of serum OPN F and P were 0.4602 and 0.006 (P = 0.05) respectively in the four groups, the differences were statistically significant, so the serum OPN levels were significantly different among the four groups.In view of this difference, we further performed multiple comparisons among the four groups.The results showed that the serum OPN levels in patients with Hashimoto's thyroiditis were significantly higher than those in the other three groups. 2. There was no significant difference between IL-33, OPN and SLE disease activity.There was no correlation between serum IL-33 and OPN levels.The level of serum IL-33 was higher in patients with anti SSA-Ab positive SLE, but there was no correlation between serum OPN level and clinical manifestations. There was no significant difference between serum OPN level and disease activity of SS (except anti SSB-Ab positive patients).Most clinical and laboratory changes were not associated with serum IL-33 and OPN levels.The serum OPN level was higher in SS patients with anti SSB-Ab positive, but there was no correlation between serum IL-33 level and clinical manifestations.[conclusion] there was no significant difference in serum IL-33 level between SLESS-SLE patients with Hashimoto's thyroiditis and normal controls, but the serum OPN level was significantly higher in patients with OPN complicated with Hashimoto's thyroiditis than in the other three groups.
【学位授予单位】：昆明医科大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R593.2

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