实验性自身免疫性重症肌无力模型大鼠腓肠肌低密度脂蛋白受体相关蛋白4表达的特点

发布时间：2018-04-19 11:07

本文选题：低密度脂蛋白受体相关蛋白 + 重症肌无力　；参考：《临床神经病学杂志》2017年02期

【摘要】：目的探讨实验性自身免疫性重症肌无力(EAMG)模型大鼠腓肠肌低密度脂蛋白受体相关蛋白4(LRP4)表达的特点。方法将30只大鼠随机分为EAMG组、对照组和空白组。以小鼠乙酰胆碱受体(AChR)基因为模板合成AChR抗原,以Lewis大鼠为免疫动物。EAMG组采取主动免疫法将AChR抗原与弗氏佐剂混合制成乳剂,注射入大鼠背部皮下。对照组在相同部位注射等量的弗氏佐剂。空白组在相同部位注射等量的生理盐水。8周后使用低频重复电刺激(RNS)检测肌电RNS衰减率。采用蛋白免疫印迹法检测腓肠肌LRP4含量。结果 EAMG组所有大鼠在8周后出现肌无力症状,对照组、空白组大鼠无肌无力症状。EAMG组大鼠RNS衰减率明显高于对照组和空白组(均P0.05)。EAMG组大鼠腓肠肌LRP4蛋白的相对含量明显低于对照组和空白组(均P0.05)。结论 EAMG大鼠中,当AChR被自身抗体破坏时,可能导致包含LRP4在内的整条信号通路受损,进而使LRP4含量减少。LRP4是神经肌肉接头信号通路完整的重要分子。
[Abstract]:Objective to investigate the expression of low density lipoprotein receptor associated protein (LRP4) in gastrocnemius muscle of experimental autoimmune myasthenia gravis rats.Methods 30 rats were randomly divided into EAMG group, control group and blank group.The AChR antigens were synthesized from the acetylcholine receptor (ache) group of mice. The Lewis rats were used as immune animals. The EAMG group prepared the emulsion with the mixture of AChR antigen and Freund's adjuvant by active immune method, and injected into the back of the rats subcutaneously.The control group received the same dose of Freund's adjuvant at the same site.Low frequency repetitive electrical stimulation (RNS) was used to detect the RNS attenuation rate in the blank group after injection of the same amount of normal saline at the same site for 8 weeks.The content of LRP4 in gastrocnemius muscle was detected by Western blot.Results all the rats in EAMG group developed myasthenia after 8 weeks.The attenuation rate of RNS in control group and blank group was significantly higher than that in control group and blank group. The relative content of LRP4 protein in gastrocnemius muscle in P0.05).EAMG group was significantly lower than that in control group and blank group (all P 0.05).Conclusion in EAMG rats, when AChR is destroyed by autoantibodies, the whole signal pathway including LRP4 may be damaged, and the decrease of LRP4 content. LRP4 is an important molecule in the complete signal pathway of neuromuscular junction.
【作者单位】：河南省濮阳市人民医院神经内科;河北医科大学第二附属医院神经内科;
【分类号】：R746.1;R-332

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