成骨细胞分化、骨形成与修复中转录因子Osx和Satb2的调控作用

发布时间：2018-04-20 01:03

本文选题：骨质疏松 + 转录因子　；参考：《中国组织工程研究》2016年07期

【摘要】：背景:成骨细胞主要来自于骨髓细胞向骨基质中的间充质细胞,一些转录因子或局部因素可促进骨髓基质细胞调节分化为成骨细胞。目的:明确C_2C_12细胞以及Osx与Satb2在骨质疏松修复过程中的作用。方法:取野生型SD大鼠20只,分为正常组10只,假手术组和骨质疏松模型组(模型组)各5只,同时取Osx-KO大鼠10只。模型组和Osx-KO大鼠切除双侧卵巢构建野生型大鼠与Osx-KO大鼠进行骨质疏松模型;假手术组找出双侧卵巢但不切除。检测各组大鼠术后体质量的变化及股骨骨密度含量。体外培养C_2C_12细胞,并设计了si RNA-Satb2、si RNA-Osx,通过细胞实验、基因沉默、western blot法,观察成骨细胞分化的相关Osx与Satb2的表达及对骨质疏松的影响。结果与结论:(1)体质量:造模12周后,模型组、Osx-KO组大鼠较正常组和假手术组大鼠显著增加(P0.01)。(2)骨密度:模型组、Osx-KO组较正常组和假手术组显著降低(P0.01)。(3)转录因子:野生型大鼠各因子均有表达,在Osx-KO大鼠中,Satb2、Osx基因的表达显著降低(P0.001),而Runx2基因在两种类型的大鼠中的表达差异无显著性意义。(4)当对基因进行沉默后:再检测相关的成骨基因发现Satb2、Osx、Runx2、ALP基因的表达水平均有显著的抑制。(5)结果说明:在骨质疏松发病中转录因子Osx、Satb2可能是保护性分子,对成骨细胞分化、骨形成与修复有着关键的调控作用。
[Abstract]:Background: osteoblasts mainly come from bone marrow cells to mesenchymal cells in bone matrix. Some transcription factors or local factors can promote the regulation and differentiation of bone marrow stromal cells into osteoblasts. Objective: to investigate the role of C_2C_12 cells and Osx and Satb2 in the repair of osteoporosis. Methods: twenty wild Sprague-Dawley rats were divided into normal group (n = 10), sham operation group (n = 5) and osteoporosis model group (n = 5), and Osx-KO rats (n = 10). Osteoporosis model of wild type rats and Osx-KO rats was established in the model group and Osx-KO rats by bilateral ovariectomy, while in the sham-operation group, bilateral ovaries were found out but not resected. The changes of body mass and bone mineral density of femur were measured. C_2C_12 cells were cultured in vitro, and si RNA-Satb2 siRNA-Osx was designed. The expression of Osx and Satb2 in osteoblast differentiation and the effect on osteoporosis were observed by cell experiment and gene silencing western blot method. Results and conclusion: body mass: 12 weeks later, The bone mineral density (BMD) of the model group was significantly higher than that of the normal group and the sham-operated group. The expression of all the factors in the wild type rats was significantly lower than that in the model group compared with the normal group and the sham operation group, and that in the model group was significantly lower than that in the normal group and the sham-operated group, and that in the model group was significantly lower than that in the control group and the sham-operated group. In Osx-KO rats, the expression of Satb2Osx gene decreased significantly, while the expression of Runx2 gene in two types of rats showed no significant difference. The results showed that the transcriptional factor OsxanSatb2 may be a protective molecule in the pathogenesis of osteoporosis. It plays a key role in the regulation of osteoblast differentiation, bone formation and repair.
【作者单位】：广州中医药大学;广西中医药大学附属第三医院;广东省中医院;
【分类号】：R580

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