Th17细胞及相关细胞因子在AITD发病机制中的作用研究

发布时间：2018-04-20 01:27

本文选题：自身免疫性甲状腺疾病 + Th17细胞　；参考：《青岛大学》2015年硕士论文

【摘要】：目的研究初发AITD患者和正常人外周血中Th17细胞及相关细胞因子IFN-γ、IL-6、IL-17、IL-23和TGF-β1的水平变化,探讨其与甲状腺功能指标和自身免疫性抗体之间的关系,以了解其在AITD发病机制中的作用。方法对23例初发Graves病患者(GD组)、23例初发桥本甲状腺炎患者(HT组)以及23名健康对照者(NC组)的外周血分别用ELISA法检测IFN-γ、IL-6、IL-17、IL-23和TGF-β1浓度,用ECLIA法检测FT3、FT4、TSH、TPOAb和TGAb水平,用RRA法测定Tr Ab水平,用FCM检测PBMC中CD4+IL-17+T细胞比例。结果⑴,GD组和HT组的外周血中Thl7细胞比例均高于NC组(P0.01),且HT组高于GD组(P0.01);GD组的Th17细胞比例与FT3、FT4呈显著正相关(rs0.4771;P均0.05);HT组的Th17比例与Tg Ab、TPOAb呈显著正相关(rs0.5083;P均0.05)。⑵,GD组的IFN-γ、IL-6、IL-17、IL-23和TGF-β1的浓度均显著高于NC组(P均0.01);GD组和NC组的5种细胞因子水平与FT3、FT4、TPOAb、Tr Ab、Tg Ab均呈正相关(rs0.3019,P均0.05);与TSH均呈负相关(rs-0.4451,P均0.05)。⑶,HT组的IFN-γ、IL-6、IL-17和IL-23的浓度均高于NC组(P均0.05),且IL-23浓度高于GD组(P0.01);但HT组的TGF-β1浓度与NC组无差异(P0.05);相关性分析显示:HT组的IL-17与FT4呈负相关(r=-0.4135,P0.01),与TSH、TPOAb、Tg Ab、Tr Ab均呈正相关(rs0.3808;P均0.01);IL-6、IFN-γ与TPOAb均呈正相关(rs0.3399,P均0.05);IL-23与FT3、FT4均呈负相关(rs-0.4157;p均0.05),与TSH、Tg Ab、TPOAb均呈正相关(rs0.4325;p均0.05)。结论Thl7细胞及相关细胞因子IFN-γ、IL-6、IL-17、IL-23等在初发GD或HT患者外周血中均高表达;TGF-β1仅在初发GD患者血清中高表达;Thl7细胞及相关细胞因子与初发AITD患者的甲状腺激素和自身抗体水平密切相关。Thl7细胞及相关细胞因子是介导AITD发病的重要分子机制,并与AITD病情的轻重程度有关。
[Abstract]:Objective to study the changes of the levels of Th17 cells and the related cytokines IFN- 纬 -6, IL-17, IL-23 and TGF- 尾 1 in peripheral blood of patients with primary AITD and normal controls, and to explore the relationship between the levels of IL-23 and TGF- 尾 1, thyroid function and autoimmune antibodies, in order to understand their role in the pathogenesis of AITD. Methods the levels of IL-23 and TGF- 尾 1 in peripheral blood of 23 patients with primary Graves's disease and 23 patients with primary Hashimoto's thyroiditis (HT group) and 23 healthy controls (NC group) were detected by ELISA, and the levels of FT3T4TSHTPOAb and TGF- 尾 1 were detected by ECLIA method. The level of Tr Ab was measured by RRA and the proportion of CD4 IL-17 T cells in PBMC was detected by FCM. Results 1 the percentage of Thl7 cells in peripheral blood of GD group and HT group was higher than that of NC group P0.01G, and the proportion of Th17 cells in GD group was significantly higher than that in GD group, and there was a significant positive correlation between the proportion of Th17 cells in GD group and FT3T4 in GD group. The concentrations of IL-23 and TGF- 尾 _ 1 in IL-6, IL-17 and TGF- 尾 1 were significantly higher than those in GD group and NC group, and the levels of 5 cytokines were positively correlated with FT _ 3T _ 4T _ (4) TPO _ (4) TPO _ (1) T _ (+) T _ (+) T _ (+) T _ (+) ~ (0.3019) P, and were negatively correlated with TSH (r ~ (- 纬) -0.4451p, 0.05 ~ (.5)) and the concentrations of IL-6IL-17 and IL-23 were all higher than those of NC group (P 0.05), and the correlation between IL-23 and TGF- 尾 _ (1) was significantly higher than that in NC group (P = 0.05). The concentration of IL-23 was higher than that of GD group (P 0.01), but the concentration of TGF- 尾 1 in HT group was not different from that in NC group (P 0.05). The correlation analysis showed that IL-17 in the group of 10 HT had a negative correlation with FT4, and there was a positive correlation between the concentration of TGF- 尾 _ 1 and FT4, and the positive correlation between TGF- 尾 _ 1 and FT4, and the positive correlation between TGF- 尾 _ 1 and TPOAb, r _ S _ (0.339N) P _ (0.05) and FT _ (3) T _ (4) in HT group, and positive correlation between TGF- 尾 _ 1 and TGF- 尾 _ (1) in HT group and TPOAb in T _ (3) T _ (3) T _ (4). There was a positive correlation between rs-0.4157p and TSHG Ab-TPOAb, and there was a positive correlation between rs0.4157 and TPOAb. Conclusion the high expression of TGF- 尾 1 in the peripheral blood of patients with GD or HT is only in the serum of patients with primary GD and related cytokines, and the expression of TGF- 尾 1 is only in the serum of patients with primary GD. The expression of TGF- 尾 1 and the associated cytokines in the serum of patients with primary GD and the thyroid hormone and autoantibodies in patients with primary AITD are higher than those in the patients with primary AITD. Body level is closely related to. Thl7 cells and related cytokines are important molecular mechanisms that mediate the pathogenesis of AITD. And it was related to the severity of AITD.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R581

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