运动对废用性骨质疏松大鼠骨代谢和破骨细胞分化OPG-RANKL-RANK系统的影响

发布时间：2018-04-20 06:31

本文选题：运动 + 废用性骨质疏松　；参考：《中国运动医学杂志》2017年07期

【摘要】：目的:研究跑台运动对废用性骨质疏松的作用以及破骨细胞分化OPG-RANKL-RANK系统在其中的介导作用。方法:6周龄雄性Sprague-Dawley大鼠40只,分为正常对照组、废用模型组、正常恢复组和运动恢复组,每组10只。正常对照组不做任何特殊处理,安静饲养4周后处死;废用模型组尾部悬吊4周后处死;正常恢复组尾部悬吊4周后,安静饲养4周处死;运动恢复组尾部悬吊4周后,跑台训练4周处死。各组大鼠处死后立即进行骨密度(BMD)、骨组织形态计量学、骨组织抗酒石酸酸性磷酸酶5b(TRACP-5b)染色计量、骨代谢以及破骨细胞分化OPG-RANKL-RANK系统相关细胞因子等指标的测试。结果:废用模型组大鼠BMD、骨小梁体积百分比(TBV)、骨小梁宽度(Tb.Wi)、血清碱性磷酸酶(ALP)浓度、骨髓细胞因子OPG基因表达量均显著小于正常对照组(P0.01),而骨小梁间距(Tb.Sp)、胫骨干骺端TRACP-5b平均阳性染色面积百分比(APSAP)、血清Ca2+和TRACP-5b浓度、骨髓细胞因子RANKL、M-CSF、RANK、IL-6和TNF-α基因表达量则显著大于正常对照组(P0.01)。运动恢复组大鼠BMD、TBV、Tb.Wi、血清ALP浓度、骨髓细胞因子OPG基因表达量均显著大于正常恢复组(P0.05或P0.01),而Tb.Sp、血清Ca2+和TRACP-5b浓度、胫骨干骺端APSAP、骨髓细胞因子RANKL、M-CSF、RANK、IL-6和TNF-α基因表达量均显著小于正常恢复组(P0.05,P0.01)。结论:4周的尾部悬吊可导致大鼠废用性骨质疏松症的发生,跑台运动可以促进废用性骨质疏松症的恢复,这均与骨髓微环境破骨细胞分化OPG-RANKL-RANK系统相关细胞因子的表达有关。
[Abstract]:Aim: to study the effect of treadmill exercise on waste osteoporosis and the mediated effect of osteoclast differentiation OPG-RANKL-RANK system. Methods 40 male Sprague-Dawley rats aged 6 weeks were divided into normal control group, waste model group, normal recovery group and exercise recovery group with 10 rats in each group. The normal control group was killed after 4 weeks of quiet feeding without any special treatment; the waste model group was killed after 4 weeks of tail suspension; the normal recovery group was put to death after 4 weeks of tail suspension, and the exercise recovery group was killed after 4 weeks of tail suspension. They were executed after 4 weeks of treadmill training. Bone mineral density (BMD), bone histomorphometry, bone tissue tartrate-resistant acid phosphatase (5bAP-5b) staining, bone metabolism and cytokines related to OPG-RANKL-RANK system of osteoclast differentiation were measured immediately after the rats were killed in each group. Results: BMD, trabecular volume percentage, bone trabecular width and serum alkaline phosphatase (ALP) concentration were measured in the model group. The expression of bone marrow cytokine OPG gene was significantly lower than that of the normal control group (P 0.01), while the trabecular spacing, the average positive staining area of tibial metaphyseal TRACP-5b and the concentration of serum Ca2 and TRACP-5b were significantly higher than those in the control group. The expression of RANKL- M-CSFU, RANK- 6 and TNF- 伪 in bone marrow cells was significantly higher than that in normal control group (P 0.01). In exercise recovery group, the concentration of serum ALP and the expression of bone marrow cytokine OPG gene were significantly higher than those of normal recovery group (P0.05 or P0.01), while Tb.Sp, serum Ca2 and TRACP-5b levels were significantly higher in exercise recovery group than those in normal recovery group. The expressions of APSAP, RANKLM-CSFU, IL-6 and TNF- 伪 in tibial metaphysis were significantly lower than those in normal recovery group (P 0.05 and P 0.01). Conclusion tail suspension for 4 weeks may lead to the occurrence of abandoned osteoporosis in rats, and treadmill exercise can promote the recovery of abandoned osteoporosis, which is related to the expression of cytokines related to OPG-RANKL-RANK system of osteoclast differentiation in bone marrow microenvironment.
【作者单位】：齐鲁师范学院体育学院;
【基金】：国家体育总局全民健身研究领域课题(2015B059);国家体育总局体育哲学社会科学研究项目(2292SS16029)
【分类号】：R580

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