负载CGRP及SP的明胶缓释微球用于修复家兔骨质疏松模型骨缺损的研究

发布时间：2018-04-20 14:39

本文选题：降钙素基因相关肽 + P物质　；参考：《口腔医学研究》2016年03期

【摘要】：目的:将负载不同浓度的降钙素基因相关肽(CGRP)及P物质(SP)的明胶缓释微球植入骨质疏松兔的股骨缺损中,观察其对骨缺损修复的影响。方法:通过去势方法建立兔骨质松模型,利用乳化交联的方法制备负载不同浓度的CGRP及SP的明胶缓释微球。将24只骨质疏松兔随机分组,于股骨髁部制备骨缺损并分别植入负载10-6 mol/L、10-8 mol/L和10-10 mol/L的SP以及CGRP的明胶微球,3个月后处死观察结果。结果:负载不同浓度的CGRP及SP均能有效地促进成骨,影像学结果示SP在10-6 mol/L时其骨体积分数最高,并且能有效提高骨小梁数量,降低骨小梁间的间距(P0.05),CGRP在浓度为和10-8 mol/L时效果即达到上述效果;而组织学结果示SP和CGRP均在浓度为10-6 mol/L时其新生骨组织比例最高(P0.05)。结论:负载不同浓度的CGRP及SP均能有效地促进骨质疏松兔骨缺损的成骨,在所研究的浓度中,高浓度的效果优于低浓度的效果。
[Abstract]:Aim: to investigate the effects of gelatin sustained-release microspheres loaded with calcitonin gene-related peptide (CGRP) and substance P (SP) on bone defect in osteoporotic rabbits. Methods: rabbit bone loose model was established by castration. Gelatin sustained-release microspheres loaded with different concentrations of CGRP and SP were prepared by emulsification and crosslinking. Twenty-four osteoporotic rabbits were randomly divided into two groups. Bone defects were prepared at the femoral condyle and implanted with 10 -6 mol / L 10 ~ (-8) mol/L and 10 ~ (-10) mol/L SP and CGRP gelatin microspheres respectively. The results were observed 3 months later. Results: both CGRP and SP loaded with different concentrations could effectively promote osteogenesis. Imaging results showed that SP had the highest bone volume fraction at 10-6 mol/L and could effectively increase the number of trabeculae. The results of histopathology showed that the ratio of SP and CGRP was the highest when the concentration of P0.05 mol/L was 10 ~ (-8) mol/L and that of CGRP was 10 ~ (-6) mol/L. Conclusion: loading different concentrations of CGRP and SP can effectively promote the osteogenesis of bone defect in osteoporotic rabbits, and the effect of high concentration is better than that of low concentration.
【作者单位】：第四军医大学军事口腔医学国家重点实验室口腔外科;
【基金】：国家自然科学基金项目(编号:81271103)
【分类号】：R580;R-332

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