XCT-790对沉默DKK1、Sost腺病毒载体转染MG63细胞中OPG、CTGF、FGF2、TNFα的影响研究

发布时间：2018-04-21 04:19

本文选题：XCT- + Dickkopf　；参考：《中国骨质疏松杂志》2017年07期

【摘要】：目的研究ERRα抑制剂(XCT-790)对沉默Wnt信号通路抑制因子Dickkopf1(DKK1)、骨硬化蛋白Sclerostin(Sost)重组腺病毒载体转染MG63细胞中OPG、CTGF、FGF2、TNFα的影响。方法本实验将培养好的MG63细胞分为空白对照组、沉默DKK1组、沉默Sost组、沉默(DKK1+Sost)组、XCT-790处理空载腺病毒组、XCT-790处理沉默DKK1组、XCT-790处理沉默Sost组、XCT-790处理沉默(DKK1+Sost)组,根据组别不同分别用包装好的沉默DKK1、Sost腺病毒载体转染MG63细胞,应用Western blot检测MG63细胞中OPG、CTGF、FGF2、TNFα蛋白的表达量。结果 (1)与空白对照组对比,沉默DKK1、Sost、DKK1+Sost可以提高OPG、CTGF、FGF2表达量(P0.05),降低TNFα表达量(P0.05);XCT-790干预MG63细胞可降低OPG、CTGF、FGF2表达量(P0.05),增加TNFα表达量(P0.05);(2)与沉默DKK1、Sost、DKK1+Sost组比较,XCT-790处理沉默DKK1、Sost、DKK1+Sost组可降低因沉默DKK1、Sost、DKK1+Sost而增加OPG、CTGF、FGF2的表达量的作用(P0.05),增加因沉默DKK1、Sost、DKK1+Sost而降低TNFα的表达量(P0.05)。结论 XCT-790可以降低MG63细胞中OPG、CTGF、FGF2表达量,升高TNFα表达量;还可以调节因沉默DKK1、Sost、DKK1+Sost而改变的MG63细胞中OPG、CTGF、FGF2及TNFα的表达量。
[Abstract]:Objective to study the effect of ERR 伪 inhibitor XCT-790 on the expression of Wnt signaling inhibitor Dickkopf1, bone sclerosing protein (Sclerostintin) recombinant adenovirus vector in MG63 cells. Methods the cultured MG63 cells were divided into three groups: blank control group, silencing DKK1 group, silencing Sost group, silencing DKK1 Sostgroup (XCT-790) group. XCT-790 group treated with silent DKK1 group (XCT-790) treated silencing Sost group (XCT-790) group treated with silencing DKK1 Sostgroup. MG63 cells were transfected with adenovirus vector of silencing DKK1Sost according to different groups, and the expression of TNF- 伪 protein in MG63 cells was detected by Western blot. Results 1) compared with the blank control group, The silencing of DKK1 Sost could increase the expression of P0.05, and decrease the expression of TNF 伪 in MG63 cell line P0.05, increase the expression of TNF 伪 and increase the expression of TNF 伪 in MG63 cells. Compared with the group of DKT-790, XCT-790 could decrease the expression of TNF 伪 and increase the expression of P0.05FGF2.) compared with DKT-790 group, XCT-790 could decrease the expression of DKK1SostDK1 Sost and increase the expression of TNF 伪 in DKK1SostDK1 Sost group, which could reduce the increase of OPGCTGFF2 expression due to the silencing of DKK1 / DKK1 / DKK1 / DKK1 / DK1 / DKK1 / DK1 / DK1 / DK1 / DK1 / DK1 / DK1 / DK1 / DK1 / DK1 / DK1 / DK1 / DK1 / DK1 / MG63 cells. The effect of DK1 on the expression of TNF 伪 was increased, and the expression of TNF 伪 was decreased by silencing DK1 / DKK1 Sost. Conclusion XCT-790 can decrease the expression of FGF2 and increase the expression of TNF 伪 in MG63 cells, and can also regulate the expression of FGF2 and TNF 伪 in MG63 cells.
【作者单位】：广州中医药大学;广州中医药大学附属骨伤科医院骨科;
【基金】：国家自然科学基金(81302991,81673786) 广东省自然科学基金(S2013040016828,2014A030310127) 广东省科技计划项目(2013B021800058) 广州中医药大学青年英才项目(QNYC20120119) 2014广东省“优青计划”项目(yq2014041)
【分类号】：R580

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