薄荷醇下调肺组织P物质改善哮喘气道炎症及气道高反应性的研究

发布时间：2018-04-21 16:16

本文选题：哮喘 + 薄荷醇　；参考：《第三军医大学学报》2017年22期

【摘要】：目的探讨雾化吸入薄荷醇对哮喘小鼠气道炎症及气道高反应性的作用及其对肺组织P物质(substance P,SP)含量的影响。方法按随机数字表法将30只6~8周龄BALB/c雌鼠分为对照组、哮喘组及薄荷醇治疗组,哮喘组和薄荷醇治疗组用鸡卵清蛋白(ovalbumin,OVA)致敏激发建立哮喘模型,薄荷醇治疗组于每次雾化激发前给予1.6%薄荷醇溶液5 mL雾化吸入30 min,连续10 d,对照组用PBS替代。于末次雾化24 h内行肺功能检测小鼠气道高反应性;48 h内灌取支气管肺泡灌洗液(bronchoalveolar lavage fluid,BALF)计数细胞总数及细胞分类;肺组织HE染色观察肺部病理改变;ELISA检测血清总免疫球蛋白E(IgE)水平及肺匀浆SP含量;免疫组化染色检测肺组织SP的表达。结果哮喘组气道高反应性明显高于对照组(P0.05),而薄荷醇治疗组较哮喘组显著降低(P0.05);哮喘组BALF细胞总数及嗜酸性粒细胞绝对计数均较对照组明显增加(P0.01),薄荷醇治疗组较哮喘组显著减少(P0.01);HE染色显示,哮喘组支气管和血管周围炎症细胞浸润明显,薄荷醇治疗组较哮喘组明显减轻;ELISA结果显示,哮喘组血清总IgE水平及肺匀浆SP含量均较对照组升高(P0.01),薄荷醇治疗组较哮喘组减轻(P0.05);免疫组化染色结果显示,哮喘组肺组织SP在气道上皮细胞及血管周围强阳性表达,薄荷醇治疗组中度阳性表达。结论雾化吸入薄荷醇能减轻哮喘小鼠气道炎症及降低气道高反应性,可能与减少SP含量进而减轻肺部神经源性炎症相关。
[Abstract]:Objective to investigate the effects of nebulized menthol inhalation on airway inflammation and airway hyperresponsiveness in asthmatic mice and the effect of nebulized menthol inhalation on substance P substance (SP) content in lung tissue. Methods Thirty female BALB/c rats aged 6 and 8 weeks were randomly divided into control group, asthmatic group and menthol treatment group, asthma group and menthol treatment group, which were sensitized with ovalbumin ovalbumin OVA (ovalbumin OVA) to establish asthma model. The menthol treatment group was given 1.6% menthol solution 5 mL atomization inhalation for 30 min for 10 days before each atomization excitation. The control group was replaced by PBS. Lung function was measured within 24 h after the last atomization. The total number of cells and cell classification of bronchoalveolar lavage fluidge were collected from bronchoalveolar lavage fluid (bronchoalveolar lavage) in 48 h. The level of serum total immunoglobulin (IgE) and the content of SP in lung homogenate were detected by Elisa and the expression of SP in lung tissue was detected by immunohistochemical staining. Results the airway hyperresponsiveness in asthma group was significantly higher than that in control group (P 0.05), while in menthol group it was significantly lower than that in asthma group, while the total number of BALF cells and the absolute count of eosinophils in asthma group were significantly higher than those in control group (P 0.01) and menthol treatment group. Compared with the asthmatic group, the HE staining of P0. 01 was significantly reduced, The infiltration of inflammatory cells around the bronchi and blood vessels was obvious in asthma group, and the results of Elisa showed that menthol treatment group was significantly less than that of asthma group. The levels of serum total IgE and lung homogenate SP in asthma group were significantly higher than those in control group, and those in menthol group were lower than those in asthma group, and the results of immunohistochemical staining showed that SP was strongly expressed in airway epithelial cells and perivascular tissues in asthmatic group. Moderate positive expression in menthol treatment group. Conclusion inhalation of menthol can reduce airway inflammation and airway hyperresponsiveness in asthmatic mice, which may be related to the reduction of SP content and the reduction of pulmonary neurogenic inflammation.
【作者单位】：重庆医科大学附属儿童医院呼吸科儿童发育疾病研究教育部重点实验室儿童发育重大疾病国家国际科技合作基地儿科学重庆市重点实验室;
【基金】：国家自然科学基金面上项目(81670018);国家自然科学基金青年科学基金项目(81600022)~~
【分类号】：R562.25

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