Apelin对葡萄糖的毒性作用及对胰岛细胞PDX-1表达的影响

发布时间：2018-04-21 17:44

本文选题：Apelin + 胰十二指肠同源盒蛋白-　；参考：《重庆医学》2016年33期

【摘要】：目的探讨Apelin对葡萄糖毒性作用及对胰岛细胞胰十二指肠同源盒蛋白-1(PDX-1)表达的影响。方法在不同葡萄糖浓度下(正糖组5.6mmol/L,高糖组16.7mmol/L,极高糖组33.3mmol/L),+/-Apelin-36培养胰岛β细胞株NIT-1细胞3d,然后测定基础和葡萄糖刺激后胰岛细胞胰岛素分泌量,测定细胞内胰岛素含量和PDX-1蛋白及mRNA表达。结果与正糖组比较,高糖组和极高糖组的基础和葡萄糖刺激后胰岛素分泌、细胞内胰岛素均明显减少,PDX-1蛋白表达下降(P0.05);与未加Apelin组比较,加Apelin高糖组和极高糖组的基础和葡萄糖刺激后胰岛素分泌、细胞内胰岛素明显减少,PDX-1蛋白表达下降(P0.05);6组胰岛细胞的胰岛素水平与PDX-1蛋白表达呈正相关,与PDX-1mRNA表达无关。结论 Apelin可能通过降低PDX-1蛋白表达参与葡萄糖毒性作用,引起胰岛素分泌减少,从而在糖尿病的发病机制中发挥作用。
[Abstract]:Objective to investigate the effects of Apelin on glucose toxicity and on the expression of pancreaticoduodenal homobox protein-1 PDX-1 in islet cells. Methods islet 尾 cell line NIT-1 cells were cultured at different glucose concentrations (5.6 mmol / L in normal glucose group, 16.7 mmol / L in high glucose group, 33.3 mmol / L in very high glucose group, r / -Apelin-36 for 3 d). Insulin secretion of islet cells was measured after basal and glucose stimulation. Insulin content, PDX-1 protein and mRNA expression were measured. Results compared with the normal glucose group, the basal and glucose-stimulated insulin secretion of the high glucose group and the extremely high glucose group decreased significantly the expression of P0.05 and the expression of p0.05 protein in the cells, and compared with the control group without Apelin. The basal and glucose-stimulated insulin secretion in the Apelin high glucose group and the extremely high glucose group significantly decreased the expression of pPDX-1 protein. The insulin level of islet cells in the P0.05 group was positively correlated with the expression of PDX-1 protein, but had no correlation with the expression of PDX-1mRNA. Conclusion Apelin may play an important role in the pathogenesis of diabetes mellitus by decreasing the expression of PDX-1 protein and inducing the decrease of insulin secretion.
【作者单位】：广西医科大学第一附属医院内分泌代谢病科;
【基金】：广西壮族自治区卫生厅重点课题(桂卫重2010024)
【分类号】：R587.1

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