丁酸通过增强肝线粒体功能缓解高脂诱导的小鼠肥胖

发布时间：2018-04-25 10:08

本文选题：丁酸 + 肥胖　；参考：《中国生物化学与分子生物学报》2017年12期

【摘要】：丁酸可以预防高脂日粮诱导的小鼠肥胖和胰岛素抵抗,但是否有治疗作用尚不清楚。本研究证明,高脂日粮诱导小鼠肥胖模型后,用80 mg/mL丁酸钠水溶液灌胃能够缓解肥胖。表观指标检测发现,丁酸钠显著降低肥胖小鼠的肝重(1.24 g±0.03 g至1.08 g±0.04 g)、体重(32.46 g±0.50 g至28.35 g±0.58 g)和附睾脂重(1.33 g±0.13 g至0.81 g±0.08 g)及其与体重的比(4.06%±0.37%至2.83%±0.22%)。葡萄糖耐受实验和血液激素含量检测表明,丁酸钠部分缓解由高脂引起的葡萄糖不耐受,并显著降低血液中瘦素(3.71 ng/mL±0.62 ng/mL至1.50ng/m L±0.26 ng/mL)和胰岛素(2.39 ng/mL±0.30 ng/mL至1.25 ng/mL±0.09 ng/mL)的水平。肝中脂质和糖原的生化检测表明,丁酸钠对肝中的甘油三酯、胆固醇和糖原的含量没有显著影响。通过RT-PCR实验发现,丁酸钠显著上调线粒体β氧化和解耦联相关的关键基因以及线粒体自身编码的8个基因的mRNA水平的表达,Western印迹检测表明,丁酸钠显著升高肝葡萄糖转运蛋白GLUT2和调控线粒体功能的关键蛋白PGC-1α的表达。上述结果提示,丁酸钠可能通过增强肝线粒体功能缓解食源性小鼠肥胖。
[Abstract]:Butyric acid can prevent obesity and insulin resistance induced by high fat diet in mice. This study demonstrated that the obesity model of mice induced by high fat diet could be alleviated by oral administration of 80 mg/mL sodium butyrate solution. It was found that sodium butyrate significantly decreased the liver weight of obese mice from 1.24 g 卤0.03 g to 1.08 g 卤0.04 g, weight of 32.46 g 卤0.50 g to 28.35 g 卤0.58 g) and epididymal lipid weight of 1.33 g 卤0.13 g to 0.81 g 卤0.08 g), and the ratio to body weight was 4.06% 卤0.37% to 2.83% 卤0.22g. Glucose tolerance test and determination of serum hormones showed that sodium butyrate partially alleviated glucose intolerance induced by hyperlipidemia, and significantly decreased the levels of leptin in blood from 3.71 卤0.62 ng/mL to 1.50ng/m L 卤0.26 ng / mL) and insulin (2.39 卤0.30 ng/mL to 1.25 ng/mL 卤0.09 ng / mL). Biochemical analysis of lipids and glycogen in the liver showed that sodium butyrate had no significant effect on the contents of triglycerides, cholesterol and glycogen in the liver. RT-PCR assay showed that sodium butyrate upregulated the expression of mRNA in mitochondrial 尾 -oxidation and decoupling related genes and 8 genes encoded by mitochondria. Sodium butyrate significantly increased the expression of hepatic glucose transporter GLUT2 and PGC-1 伪, a key protein regulating mitochondrial function. These results suggest that sodium butyrate may alleviate obesity by enhancing the function of liver mitochondria.
【作者单位】：南京农业大学农业部动物生理生化重点实验室;盐城师范学院海洋与生物工程学院;
【基金】：江苏省高校自然科学研究面上项目(No.17KJB230004) 国家自然科学青年基金项目(No.31402161)资助~~
【分类号】：R589.2

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