钒酸盐对Ⅰ型糖尿病大鼠骨矿盐代谢及骨显微结构的影响

发布时间：2018-04-29 05:41

本文选题：钒酸盐 + Ⅰ型糖尿病　；参考：《中国骨质疏松杂志》2017年07期

【摘要】：目的评价钒酸盐对Ⅰ型糖尿病大鼠骨代谢及骨显微结构的影响。方法 45只雄性Wistar大鼠被分为3组:正常饲养组(n=15)、高血糖组(n=15)、高血糖钒酸盐治疗组(n=15)。对高血糖组和高血糖钒酸盐治疗组大鼠进行Ⅰ型糖尿病造模,正常饲养组大鼠尾静脉注射生理盐水作为对照。以8 w龄Wistar雄性大鼠按45 mg/kg体重注射佐链尿菌素,制作糖尿病大鼠模型。造模成功后,给予高血糖钒酸盐治疗组糖尿病大鼠应用钒酸盐治疗,每只大鼠按0.18 mg/(kg·d)钒计量喂养,每只大鼠通过灌胃治疗,治疗共持续12 w。正常饲养组和高血糖组则以生理盐水灌胃进行对照。连续喂养12 w,期间每周分别对3组大鼠进行体重、血糖监测。喂养结束以后,分别取3组大鼠胫骨、股骨进行Micro-CT检测、免疫组化和组织形态学检测、生物力学检测、骨矿含量等相关指标检测。结果经过12 w治疗,结果显示钒酸盐能够明显改善大鼠的高血糖状态,提高体重指标,而且治疗组平均骨小梁厚度、最大负荷载力、骨盐含量指标均明显高于高血糖组(P0.05)。但钙盐的含量并没有显著提高(P0.05)。结论钒酸盐能够降低高血糖大鼠的血糖值,改善糖尿病大鼠的骨代谢、骨小梁显微结构及骨组织的力学性能,可为糖尿病性骨质疏松症提供新的治疗方法。
[Abstract]:Objective to evaluate the effects of vanadate on bone metabolism and bone microstructure in type 1 diabetic rats. Methods 45 male Wistar rats were divided into three groups: normal feeding group, hyperglycemia group, hyperglycemia group and hyperglycemic vanadate treatment group. Rats in hyperglycemia group and hyperglycemic vanadate group were treated with type 1 diabetes mellitus, and rats in normal feeding group were injected with normal saline through tail vein as control group. Diabetic rats were induced by injection of streptozotocin at 45 mg/kg body weight with 8 w old Wistar male rats. After the model was successfully established, the diabetic rats in the hyperglycemic vanadate group were treated with vanadate. Each rat was fed with 0.18 mg/(kg / d) vanadium, and each rat was treated by gavage for 12 weeks. The normal feeding group and hyperglycemia group were fed with normal saline. During 12 weeks of continuous feeding, three groups of rats were monitored for body weight and blood glucose. After feeding, the tibia and femur of three groups of rats were tested for Micro-CT, immunohistochemistry, histomorphology, biomechanics, bone mineral content and so on. Results after 12 weeks of treatment, vanadate could significantly improve the hyperglycemia and body weight of rats, and the mean trabecular thickness, maximum load and bone salt content in the treatment group were significantly higher than those in the hyperglycemia group (P 0.05). However, the content of calcium salt did not significantly increase the content of P0. 05%. Conclusion vanadate can decrease the blood glucose level of hyperglycemic rats, improve the bone metabolism, trabecular microstructure and mechanical properties of bone in diabetic rats. Vanadate can provide a new treatment for diabetic osteoporosis.
【作者单位】：中国医科大学肿瘤医院/辽宁省肿瘤医院骨与软组织肿瘤科;
【分类号】：R580;R587.2

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