鼠李糖乳杆菌改善卵清蛋白诱导的食物过敏小鼠症状及其机制

发布时间：2018-04-29 20:28

本文选题：鼠李糖乳杆菌 + 卵清蛋白(OVA)　；参考：《细胞与分子免疫学杂志》2017年05期

【摘要】：目的考察鼠李糖乳杆菌GG株(LGG)对卵清蛋白诱导的食物过敏小鼠外周血白细胞介素4(IL-4)和γ干扰素(IFN-γ)水平的影响。方法将食物过敏小鼠分为食物过敏组、LGG低剂量组[1×10~8菌落形成单位(CFU)/mL,每天每只小鼠灌胃200μL]、LGG高剂量组(1×10~9CFU/mL,每天每只小鼠灌胃200μL),以同期10只正常小鼠为对照。造模成功后开始给药,LGG连续灌胃给药22d,过敏模型组和正常小鼠连续灌胃生理盐水22d。检测23d的过敏症状,测定体质量和胸腺指数、脾脏指数,ELISA检测血清卵清蛋白(OVA)特异性IgE、IL-4和IFN-γ水平,HE染色检测小肠组织病变情况和细菌培养法检测粪便菌群的改变。结果各组小鼠的胸腺指数和脾脏指数差异不明显。与对照组比,过敏小鼠模型组IL-4水平、过敏评分、大肠杆菌和类杆菌明显增加,IFN-γ水平降低、IFN-γ/IL-4比值、IgE、体质量、双歧杆菌和乳酸杆菌明显减少;肠道黏膜坏死,部分上皮细胞水肿,坏死。与过敏小鼠模型组比,LGG处理的小鼠IL-4水平、过敏评分、大肠杆菌和类杆菌明显降低,IFN-γ水平升高、IFN-γ/IL-4比值、IgE、体质量、双歧杆菌和乳酸杆菌明显增加,且呈剂量依赖性关系;LGG小鼠肠道黏膜完整,小肠细胞排列紧密。结论LGG可显著改善OVA诱导的食物过敏小鼠的过敏症状,可能与降低外周血IL-4/IFN-γ比值有关。
[Abstract]:Objective to investigate the effects of Lactobacillus rhamnoides GG strain LGG on the levels of interleukin-4 (IL-4) and interferon 纬 (IFN- 纬) in peripheral blood of food allergy mice induced by ovalbumin (ovalbumin). Methods the mice with food allergy were divided into low dose group [1 脳 10 ~ 8 colony forming unit CFU / mL, 200 渭 L / day] LGG high-dose group (1 脳 10 ~ (9) CFU / m ~ (-1), and each mouse (n = 10) were treated with 200 渭 L 路L ~ (-1) 路L ~ (-1) 路L ~ (-1) 路g ~ (-1) 路L ~ (-1). After the establishment of the model, LGG was administered continuously for 22 days, and the allergic model group and normal mice were given saline for 22 days. The allergic symptoms, body mass and thymus index were measured for 23 days. Elisa was used to detect serum ovalbumin (OVA) -specific IgEIL-4 and IFN- 纬 levels. The pathological changes of small intestine were detected by HE staining and fecal microflora was detected by bacterial culture. Results there was no significant difference in thymus index and spleen index in each group. Compared with the control group, the levels of IL-4, allergic score, Escherichia coli and Bacteroid bacilli in the allergic mice model group were significantly increased, the ratio of IFN- 纬 / IL-4, body weight, bifidobacterium and lactobacillus were significantly decreased, intestinal mucosal necrosis and edema of some epithelial cells were observed. Necrosis. Compared with the allergic mice model group, the IL-4 level, allergic score, Escherichia coli and Bacteroid bacilli significantly decreased the level of IFN- 纬 and the ratio of IFN- 纬 / IL-4 increased, the body weight, Bifidobacterium and Lactobacillus increased significantly. In a dose-dependent manner, the intestinal mucosa of LGG mice was intact and the intestinal cells were arranged closely. Conclusion LGG can significantly improve the allergic symptoms of food allergy mice induced by OVA, which may be related to the decrease of IL-4 / IFN- 纬 ratio in peripheral blood.
【作者单位】：郑州市儿童医院儿童保健科;新乡医学院生物化学与分子生物学教研室;
【基金】：世界健康基金会“海儿童医学中心-雅培/世界健康基金会临床营养发展中心(AFINS)项目”(AFINS-HOPE-2012-04)
【分类号】：R593.1

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